Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing.

Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing. Research Abstract Details 

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Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing. Abstract Text:

S Iwasaki,J Mizuno,K Kobayashi,Y Yoshikane,T Hayashi,

Changes in the morphology and cell number of the inner cell mass (ICM) of porcine blastocysts at the expanded and hatched stages during freezing (-6.8 degrees C, -35 degrees C and -196 degrees C) were studied by differential fluorochrome staining. The shape of each ICM cell from fresh blastocysts at the expanded and hatched stages was sharply delineated but that of ICM cells from frozen blastocysts was partially distorted. The cell-to-cell contact of the ICM from fresh blastocysts was tight, while that from frozen blastocysts was loose or scattered. The percentages (18 to 38%) of expanded and hatched blastocysts with tight-contact ICM cells from frozen groups at each step were significantly lower (P<0.05) than that (100%) from fresh blastocysts. The number of live ICM cells and their proportion from frozen expanded blastocysts (10.9, 12,4% at -36 degrees C) were significantly lower (P<0.05) than those from fresh embryos (18.4, 19.1%) and at -196 degrees C (20.6, 18.4%). At the hatched stage, the number of live ICM cells and their proportion were not significantly different between each freezing step. These results show that the ICM of porcine embryos at both the expanded- and hatched-blastocysts stages survived even after freezing at -196 degrees C and that the degree of ICM damage was lower at the hatched stage than at the expanded stage.

Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing. Publishing Authors By Initials

S Iwasaki,J Mizuno,K Kobayashi,Y Yoshikane,T Hayashi,

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Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 42

Page Numbers: 841-8

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Oct

YEAR: 1994

Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Changes in morphology and cell number of inner cell mass of porcine blastocysts during freezing.

AFFILIATION: NODAI Research Institute, Tokyo University of Agriculture Setagaya-ku, Tokyo 156, Japan.

Country: United States

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MEDLINETA: Theriogenology

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