Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice.

Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Research Abstract Details 

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Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Abstract Text:

Christopher D Lascola,Allen W Song,Timothy A Haystead,David S Warner,Katleen Verleysen,Tiffany A Freed,James M Provenzale,

OBJECTIVE: Gliosis refers to a range of glial cell transformations that vary according to specific brain pathologic states. Disease, however, is not a prerequisite for gliosis because glial reactivity may also be seen in regions of increased physiologic activity. Our study tests the hypothesis that high-field-strength magnetization transfer MRI is a sensitive technique for detecting transient glial reactivity after experimental spreading depression, a relatively benign perturbation unaccompanied by cell injury. MATERIALS AND METHODS: Unilateral neocortical spreading depression was elicited in mouse cerebral hemispheres and confirmed by transcranial blood flow and extracellular potential measurements. After 3 days, mice were imaged at 4 T using magnetization transfer techniques. Astroglial reactivity was determined immunohistochemically, and protein expression in control and experimental hemispheres was compared using proteomic techniques. RESULTS: Sixteen ([mean +/- SD] +/- 3) spreading depressions (n = 10) were recorded in experimental hemispheres. Spreading depression was never observed in control hemispheres. At 3 days, an 8% decrease (p < 0.05, n = 4) in magnetization transfer signal intensity was measured in experimental hemispheres, which was associated with a 37% increase (p < 0.001, n = 4) in the intensity of glial fibrillary acidic protein staining. Proteomic analysis performed 3 days after the induction of spreading depression showed upregulation of at least 56 proteins, including extracellular and intracellular elements. CONCLUSION: Magnetization transfer at 4.0-T MRI is a sensitive method for detecting glial reactivity and changes in protein expression not associated with cell injury. These results suggest magnetization transfer MRI techniques may have potential for detecting glial reactivity in physiologic processes such as learning and in early disease states.

Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Publishing Authors By Initials

CD Lascola,AW Song,TA Haystead,DS Warner,K Verleysen,TA Freed,JM Provenzale,

For similar spreading cortical depression research abstracts see: spreading cortical depression research

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Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: AJR. American journal of roentgenology

VOLUME: 183

Page Numbers: 1791-7

Journal Abbreviation:

ISSN: 0361-803X

DAY: 15

MONTH: Dec

YEAR: 2004

Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Information

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LANGUAGE: eng

NlmUniqueID: 7708173

Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Keywords Mesh Terms:

KEYWORDS: Spreading Cortical Depression

MESH TERMS: physiology

Chemical & Substance for Abstract: Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Changes in magnetization transfer MRI correlate with spreading depression-induced astroglial reactivity and increased protein expression in mice.

AFFILIATION: Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA.

Country: United States

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MEDLINETA: AJR Am J Roentgenol

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