Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction.

Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Research Abstract Details 

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Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Abstract Text:

Protein kinase activity has been found in hepatitis B virions (Dane particles) purified from the plasma of hepatitis B surface antigen carriers [Albin, C., and Robinson, W.S. (1980) J. Virol. 34, 297-302]. Dane particles were purified from the pooled, HBeAg-positive plasma. When this preparation was incubated with [gamma 32P]ATP in the presence of 10mM MnCl2 and 0.5% NP-40 for 15 seconds at 30 degrees C, several phosphorylated polypeptides of 20,000, 42,000, 48,000, 50,000 and 56,000 daltons were detected in sodium dodecyl sulfate-polyacrylamide gels. When the Dane particles were incubated with [gamma 32P]ATP, 10 mM MnCl2, and 0.5% NP-40 in the presence of human hepatoma cell (J-5) particulate fraction at 30 degrees C, 15 seconds, the 42,000, 48,000 and 50,000 daltons phosphorylated polypeptides were not found. When human peripheral blood lymphocytes particulate fraction was incubated with Dane particles under the same conditions, no change of Dane particle phosphorylated polypeptides was detected. Previous publications [Albin, C., and Robinson, W.S. (1980) J. Virol. 34, 297-302; Gerlich, W.H. et al. (1982) J. Virol. 42, 761-766] showed that when hepatitis B core particles purified from hepatoma tissues contained protein kinase activity, only phosphorylated polypeptide was 20,000 daltons. Our data suggested that when Dane particles were put in an environment of hepatoma cells (or tissues), the protein kinase could only phosphorylate selected polypeptides in these particles.

Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Publishing Authors By Initials

For similar viruses: virion research abstracts see: viruses: virion research

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Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Science Council, Repub

VOLUME: 8

Page Numbers: 143-7

Journal Abbreviation: Proc. Natl. Sci. Counc. Repub.

ISSN: 0255-6596

DAY: 26

MONTH: Apr

YEAR: 1984

Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8502426

Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: enzymology

Chemical & Substance for Abstract: Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction. Information

Substance Name: Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Change of hepatitis B virions (Dane particles) phosphorylation pattern by human hepatoma cell particulate fraction.

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Country: TAIWAN

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MEDLINETA: Proc Natl Sci Counc Repub Chin

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