cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein.

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Abstract Text:

Jesse D Roberts,Jean-Daniel Chiche,Emily M Kolpa,Donald B Bloch,Kenneth D Bloch,Jesse D Roberts,Jean-Daniel Chiche,Emily M Kolpa,Donald B Bloch,Kenneth D Bloch,

Nitric oxide modulates vascular smooth muscle cell (SMC) cytoskeletal kinetics and phenotype, in part, by stimulating cGMP-dependent protein kinase I (PKGI). To identify molecular targets of PKGI, an interaction trap screen in yeast was performed using a cDNA encoding the catalytic region of PKGI and a human lung cDNA library. We identified a cDNA that encodes a putative PKGI-interactor that is a novel variant of TRIM39, a member of the really interesting new gene (RING) finger family of proteins. Although this TRIM39 variant encodes the NH(2)-terminal RING finger (RF), B-box, and coiled-coil (RBBC) domains of TRIM39, instead of a complete COOH-terminal B30.2 domain, this TRIM39 isoform contains the COOH-terminal portion of Rpp21, a component of RNase P. RT-PCR demonstrated that the TRIM39 variant, which we refer to as TRIM39R, is transcribed in the human fetal lung and in rat pulmonary artery SMC. Indirect immunofluorescence using an antibody generated against the conserved domains of TRIM39 and TRIM39R revealed the proteins in speckled intranuclear structures in human acute monocytic leukemia (THP-1) and human epidermal carcinoma line (HEp-2) cells. PKGI phosphorylated a typical PKGI/PKA phosphorylation domain in a conserved region of TRIM39 and TRIM39R. Additional studies demonstrated that PKGI interacts with both isoforms of TRIM39 in yeast cells and phosphorylates both isoforms of TRIM39 in human cell lines. Although PKGI has been observed to interact with proteins that regulate cytoskeletal function and gene expression, this investigation shows for the first time that PKGI interacts with tripartite motif (TRIM) proteins, which, through diverse molecular pathways, are often observed to regulate important aspects of cellular homeostasis.

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Publishing Authors By Initials

JD Roberts,JD Chiche,EM Kolpa,DB Bloch,KD Bloch,JD Roberts,JD Chiche,EM Kolpa,DB Bloch,KD Bloch,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

PUBMED ID PMID:

MEDLINE DATE:

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Lung cellular and

VOLUME: 293

Page Numbers: L903-12

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 29

MONTH: 06

YEAR: 2007

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901229

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: genetics

Chemical & Substance for Abstract: cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. Information

Substance Name: cGMP-dependent protein kinase Ialpha

Registry Number: EC 2.7.10.-

Grant and Affiliation Information for cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein.

AFFILIATION: Department of Anesthesia, Boston, Massachusetts, USA. roberts@cvrc.mgh.harvard.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL-74352

ACRONYM: HL

MEDLINETA: Am J Physiol Lung Cell Mol Phy

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: EU012025

Number Hits: 0

cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein Related Publications

• A preliminary randomized, double-blind, placebo-controlled study of the safety and efficacy of ondansetron in the treatment of cocaine dependence.
• Abnormal expression of mu-crystallin in facioscapulohumeral muscular dystrophy.
• Absence of keratin 19 in mice causes skeletal myopathy with mitochondrial and sarcolemmal reorganization.
• Controlling for occasion-specific effects when assessing the test-retest reliability of self-report health questionnaires.
• Daytime Cheyne-Stokes respiration in ambulatory patients with severe congestive heart failure is associated with increased mortality.
• Delayed Recovery Of Skeletal Muscle Function In Dysferlin- deficient Mice Following Large-strain Lengthening Contractions: 1857: Board #145 May 31 9:00 AM - 10:30 AM.
• Demonstration of presence of acanthamoeba mitochondrial DNA in brain tissue and cerebrospinal fluid by PCR in samples from a patient who died of granulomatous amebic encephalitis.
• Different obscurin isoforms localize to distinct sites at sarcomeres.
• Disruption of nitric oxide synthase 3 protects against the cardiac injury, dysfunction, and mortality induced by doxorubicin.
• Extracorporeal circulation activates endothelial nitric oxide synthase in erythrocytes.
• Influence Of An Interdisciplinary Programme Adipocytokines And Insulin Resistance In Obese Children And Adolescence: 1502: Board #265 May 30 9:30 AM - 11:00 AM.
• Mapping the binding site on small ankyrin 1 for obscurin.
• Molecular and phylogenetic analyses reveal mammalian-like clockwork in the honey bee (Apis mellifera) and shed new light on the molecular evolution of the circadian clock.
• Obscurin: a multitasking muscle giant.
• Probing the mRNA processing body using protein macroarrays and "autoantigenomics".
• Substance abuse among medical practitioners.
• THE CONTRIBUTION OF MICROSCOPES TO THE STUDY OF LIVING CIRCULATION: NEWER METHODS FOR THE STUDY OF DYNAMIC EVENTS IN LIVING ORGANS.
• Tailoring peripartum nursing care for women of advanced maternal age.
• The actin binding domain of ACF7 binds directly to the tetratricopeptide repeat domains of rapsyn.
• The art of psychiatry.
• Transzygomatic approach to the tentorial incisura: surgical anatomy.
• Value of the 12-lead resting electrocardiogram for the diagnosis of previous myocardial infarction in paced patients.
• [A new operative procedure for the treatment of urinary incontinence]
• [Consequence of GKV-WSG for physicians' remuneration. Facts--analyses--perspectives]
• [Hemoptysis after subclavian vein puncture]
• [Modalities of the early development of the embryo and nidation in mammals]
• [On the problem of gland cells in the uterine muscles in women]
• [Studies on the influence of estrogen on ovum implantation in mice]
• [Treatment of so-called functional uterine hemorrhages]
• cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein.