Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes.

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Abstract Text:

Shingo Ito,Sumio Ohtsuki,Junichi Kamiie,Yasuko Nezu,Tetsuya Terasaki,Shingo Ito,Sumio Ohtsuki,Junichi Kamiie,Yasuko Nezu,Tetsuya Terasaki,

Soluble amyloid-beta peptide (Abeta) exists in the form of monomers and oligomers, and as complexes with Abeta-binding molecules, such as low-density lipoprotein receptor-related protein-1 (LRP-1) ligands. The present study investigated the effect of self-aggregation and LRP-1 ligands on the elimination of human Abeta(1-40) [hAbeta(1-40)] from the rat brain across the blood-brain barrier. Incubation of [(125)I]hAbeta(1-40) monomer resulted in time-dependent and temperature-dependent dimer formation, and the apparent elimination rate of [(125)I]hAbeta(1-40) dimer was significantly decreased by 92.7% compared with that of [(125)I]hAbeta(1-40) monomer. Pre-incubation with LRP-1 ligands, such as activated alpha2-macroglobulin (alpha2M), apolipoprotein E2 (apoE2), apoE3, apoE4, and lactoferrin, reduced the elimination of [(125)I]hAbeta(1-40). By contrast, pre-administration of the same concentration of these molecules in the rat brain did not significantly inhibit [(125)I]hAbeta(1-40) monomer elimination. Purified [(125)I]hAbeta(1-40)/activated alpha2M complex and [(125)I]activated alpha2M were not significantly eliminated from the rat brain up to 60 min. MEF-1 cells, which have LRP-1-mediated endocytosis, exhibited uptake of [(125)I]activated alpha2M, and enhancement of [(125)I]hAbeta(1-40) uptake upon pre-incubation with apoE, suggesting that [(125)I]activated alpha2M and [(125)I]hAbeta(1-40)/apoE complex function as LRP-1 ligands. These findings indicate that dimerization and LRP-1-ligand complex formation prevent the elimination of hAbeta(1-40) from the brain across the blood-brain barrier.

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Publishing Authors By Initials

S Ito,S Ohtsuki,J Kamiie,Y Nezu,T Terasaki,S Ito,S Ohtsuki,J Kamiie,Y Nezu,T Terasaki,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 103

Page Numbers: 2482-90

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 1

MONTH: 10

YEAR: 2007

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985190

Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes.

AFFILIATION: Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai, Japan, and SORST of the Japan Science and Technology Agency, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Neurochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cerebral clearance of human amyloid-beta peptide 1-40 across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes Related Publications

• A brief history of HLA.
• A relation between calcium binding and turbidity change in isolated sarcoplasmic reticulum.
• A single human leukocyte antigen-antibody test after heart or lung transplantation is predictive of survival.
• Brain-to-blood elimination of 24S-hydroxycholesterol from rat brain is mediated by organic anion transporting polypeptide 2 (oatp2) at the blood-brain barrier.
• Cerebral clearance of human amyloid-beta peptide (1-40) across the blood-brain barrier is reduced by self-aggregation and formation of low-density lipoprotein receptor-related protein-1 ligand complexes.
• Clinical and anti-HLA antibody profile of nine renal transplant recipients with failed grafts: donor-specific and non-donor-specific antibody development.
• Comparison of focal macular cone ERGs in complete-type congenital stationary night blindness and APB-treated monkeys.
• DR antigens in bullous pemphigoid.
• Detection of HLA and MICA antibodies before kidney graft failure.
• Early evaluation of Ia monoclonal antibodies in prolonging non-human primate skin allograft survival.
• Effects of blood transfusions on renal transplants: considering exclusive blood products and donor relationship.
• Expression of nuclear receptor mRNA and liver X receptor-mediated regulation of ABC transporter A1 at rat blood-brain barrier.
• HLA class I epitopes: A and B loci.
• HLA class I epitopes: C-locus.
• HLA matching by amino acid/positions in double and triple combinations.
• HLA-DR antibody epitopes.
• Identification of Photoreceptor Precursors in the Pars Plana during Ocular Development and after Retinal Injury.
• Impact of immunosuppression on HLA-antibody formation.
• Insights into the crystal growth mechanisms of zeolites from combined experimental imaging and theoretical studies.
• Insulin facilitates the hepatic clearance of plasma amyloid beta-peptide (1 40) by intracellular translocation of low-density lipoprotein receptor-related protein 1 (LRP-1) to the plasma membrane in hepatocytes.
• Multichannel liquid chromatography-tandem mass spectrometry cocktail method for comprehensive substrate characterization of multidrug resistance-associated protein 4 transporter.
• Photopic Electroretinograms of mGluR6-Deficient Mice.
• Responsiveness to HL-A as studied by monitoring blood transfusions and kidney transplants.
• Retardation of Photoreceptor Degeneration in the Detached Retina of rd1 Mouse.
• Thirty year trend in kidney transplants: UCLA and UNOS Renal Transplant Registry.
• Unexpected frequencies of HLA antibody specificities in the sera of pre-transplant kidney patients.
• Unexpected frequencies of HLA antibody specificities present in sera of multitransfused patients.
• [Analysis of actin-binding proteins from brain using the proteomic approach]
• [Veno-arterial bypass and intraaortic balloon pumping for profound right heart failure--a case report of perioperative right heart infarction]
• mRNA expression levels of tight junction protein genes in mouse brain capillary endothelial cells highly purified by magnetic cell sorting.