Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line.

Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Research Abstract Details 

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Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Abstract Text:

Koji Toyokawa,Fabio Leite,Troy L Ott,

PROBLEM: Embryonic loss is a major contributor to infertility. Understanding factors affecting embryonic loss will help increase fertility. METHOD OF STUDY: We investigated if ovine Mx1 (oMx1) mediated secretion by ovine glandular epithelial (oGE) cells using small interfering RNA (siRNA). Effects on secretion were examined through the conventional endoplasmic reticulum-Golgi pathway using beta2- microglobulin (beta2MG) as a marker, and interferon-stimulated gene 15 (ISG15) as a marker for unconventional secretion. RESULTS: Mx1 siRNA reduced oMx1 mRNA levels at 12 and 24 hr after IFN-tau treatment (P < 0.05), without affecting levels of oMx2, ISG15, 2',5'-oligoadenylate synthetas or beta2MG. Mx1 siRNA reduced Mx1 protein levels at 48 and 120 hr after treatment (P < 0.05) and protein levels remained low at 120 hr. Transient oMx1 knock-down reduced secretion of oMx1 (P < 0.01). ISG15 protein in secretions was reduced without affecting intracellular levels (P < 0.05). Levels of beta2MG in secretions were not affected by Mx1 siRNA. CONCLUSION: We showed that oMx1 protein is secreted by oGE cells and that reduction in oMx1 protein levels by siRNA reduced secretion of ISG15, but not beta2MG. Results support the hypothesis that oMx1 is a regulator of secretion through unconventional secretory pathway(s).

Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Publishing Authors By Initials

K Toyokawa,F Leite,TL Ott,

For similar animals: chordata: vertebrates: mammals: artiodactyla: ruminants: sheep research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: ruminants: sheep research

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Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of reproductive immunology (New Y

VOLUME: 57

Page Numbers: 23-33

Journal Abbreviation: Am. J. Reprod. Immunol.

ISSN: 1046-7408

DAY: 3

MONTH: Jan

YEAR: 2007

Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8912860

Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Keywords Mesh Terms:

KEYWORDS: Sheep

MESH TERMS: genetics

Chemical & Substance for Abstract: Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line.

AFFILIATION: Department of Animal and Veterinary Science, Center for Reproductive Biology, University of Idaho, Moscow, ID, USA.

Country: Denmark

AGENCY: United States NCRR

GRANT: P20-RR15587-01

ACRONYM: RR

MEDLINETA: Am J Reprod Immunol

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