Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis.

Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Research Abstract Details 

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Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Abstract Text:

H Akiyoshi,S Fujii,

Nucleosomes isolated from Yoshida sarcoma chromatin by micrococcal nuclease treatment were relatively inactive as templates for in vitro DNA synthesis. However, the template activity increased by trypsin digestion of nucleosomes or addition of heparin to the reaction mixture. This indicates that the nucleosomal template activity is masked. A crude extract of Yoshida sarcoma cells stimulated the nucleosomal template activity. The stimulatory factor was separated into three peaks by DEAE cellulose column chromatography. The same three peaks were observed in normal rat liver extract with much lower activities, but enhanced in regenerating liver. The factors seem to stimulate DNA synthesis by activating DNA template in nucleosomes without degrading histones or changing the primary structure of nucleosomal DNA.

Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Publishing Authors By Initials

H Akiyoshi,S Fujii,

For similar genetic structures: templates, genetic research abstracts see: genetic structures: templates, genetic research

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Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 84

Page Numbers: 337-42

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1978

Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Keywords Mesh Terms:

KEYWORDS: Templates, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

Grant and Affiliation Information for Cellular factors for stimulation of nucleosomal template activity for in vitro DNA synthesis.

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Country: JAPAN

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MEDLINETA: J Biochem

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