Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB.

Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Research Abstract Details 

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Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Abstract Text:

Marc de la Roche,Amjad Mahasneh,Sheu-Fen Lee,Francisco Rivero,Graham P ,

Dictyostelium PakB, previously termed myosin I heavy chain kinase, is a member of the p21-activated kinase (PAK) family. Two-hybrid assays showed that PakB interacts with Dictyostelium Rac1a/b/c, RacA (a RhoBTB protein), RacB, RacC, and RacF1. Wild-type PakB displayed a cytosolic distribution with a modest enrichment at the leading edge of migrating cells and at macropinocytic and phagocytic cups, sites consistent with a role in activating myosin I. PakB fused at the N terminus to green fluorescent protein was proteolyzed in cells, resulting in removal of the catalytic domain. C-terminal truncated PakB and activated PakB lacking the p21-binding domain strongly localized to the cell cortex, to macropinocytic cups, to the posterior of migrating cells, and to the cleavage furrow of dividing cells. These data indicate that in its open, active state, the N terminus of PakB forms a tight association with cortical actin filaments. PakB-null cells displayed no significant behavioral defects, but cells expressing activated PakB were unable to complete cytokinesis when grown in suspension and exhibited increased rates of phagocytosis and pinocytosis.

Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Publishing Authors By Initials

M de la Roche,A Mahasneh,SF Lee,F Rivero,GP ,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins research

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Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 16

Page Numbers: 238-47

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 27

MONTH: 10

YEAR: 2004

Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Keywords Mesh Terms:

KEYWORDS: rho GTP-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB. Information

Substance Name: rho GTP-Binding Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB.

AFFILIATION: Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.

Country: United States

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MEDLINETA: Mol Biol Cell

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