Cellular control of tissue architectures using a three-dimensional tissue fabrication technique.

Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Research Abstract Details 

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Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Abstract Text:

Yukiko Tsuda,Tatsuya Shimizu,Masayuki Yamato,Akihiko Kikuchi,Tadashi Sasagawa,Sachiko Sekiya,Jun Kobayashi,Guoping Chen,Teruo Okano,Yukiko Tsuda,Tatsuya Shimizu,Masayuki Yamato,Akihiko Kikuchi,Tadashi Sasagawa,Sachiko Sekiya,Jun Kobayashi,Guoping Chen,Teruo Okano,

Tissue engineering seeks to provide regenerated tissue architectures in vitro but has not yet successfully created thick, highly vascularized, multi-functional tissues replicating native structure. We describe a novel method to fabricate pre-vascularized tissue equivalents using multi-layered cultures combining micro-patterned endothelial cells as vascular pre-cursors with fibroblast monolayer sheets as tissue matrix. Stratified tissue equivalents are constructed by alternately layering fibroblast monolayer sheets with patterned endothelial cell sheets harvested from newly developed thermo-responsive micro-patterned surfaces alternating 20 microm-wide cell-adhesive lanes with 60 microm non-adhesive zones. Cell culture substrates covalently grafted with different thermo-responsive polymers permit spatial switching of cell adhesion and detachment using applied small temperature changes. Endothelial cell patterning fidelity was maintained within the multi-layer tissue constructs after assembly, leading to self-organization into microvascular-like networks after 5-day tissue culture. This novel technique holds promise for the study of cell-cell communications and angiogenesis in reconstructed, three-dimensional environments as well as for the fabrication of tissues with complex, multicellular architecture.

Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Publishing Authors By Initials

Y Tsuda,T Shimizu,M Yamato,A Kikuchi,T Sasagawa,S Sekiya,J Kobayashi,G Chen,T Okano,Y Tsuda,T Shimizu,M Yamato,A Kikuchi,T Sasagawa,S Sekiya,J Kobayashi,G Chen,T Okano,

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Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 28

Page Numbers: 4939-46

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 20

MONTH: 08

YEAR: 2007

Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Cellular control of tissue architectures using a three-dimensional tissue fabrication technique.

AFFILIATION: Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.

Country: England

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MEDLINETA: Biomaterials

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