Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism.

Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Research Abstract Details 

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Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Abstract Text:

Veronica Paez Espinosa,Monica Ferrini,Xiaoxiong Shen,Kabirullah Lutfy,Eduardo A Nillni,Theodore C Friedman,

The prohormone convertases (PCs), PC1/3 and PC2, are involved in the tissue-specific endoproteolytic posttranslational processing of many hormonal precursors within the secretory pathway. One important prohormone, pro-thyrotropin-releasing hormone (TRH), is expressed in both hypophysiotropic (where it regulates the secretion of thyroid-stimulating hormone) and nonhypophysiotropic regions of the brain. Pro-TRH is processed at specific sites in the secretory pathway, primarily by PC1/3 followed by PC2. We hypothesized that thyroid hormone status in specific nuclei of the brain would alter pro-TRH processing by inducing changes in PC1/3 and PC2 expression. Therefore, we examined pro-TRH, PC1/3, and PC2 coexpression and coregulation in the paraventricular nucleus (PVN), lateral hypothalamus (LH), and ventromedial nucleus (VMN) of hypothyroid and euthyroid rats. Our results show that 6-n-propyl-2-thiouracil (PTU) treatment producing hypothyroidism induced a significant increase in the expression of PC1/3, PC2, and pro-TRH in the PVN and LH, but not VMN. When confocal studies were performed, an increase in colocalization of PC1/3 or PC2 in pro-TRH was observed only in PVN, a response that was especially prominent in the ventral and medial areas of the PVN. PTU did not regulate colocalization in the VMH or LH. Regulation of colocalization of processing enzyme and prohormone expression is a novel mechanism to alter hormonal biosynthesis.

Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Publishing Authors By Initials

VP Espinosa,M Ferrini,X Shen,K Lutfy,EA Nillni,TC Friedman,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research

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Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Endocrinology and

VOLUME: 292

Page Numbers: E175-86

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 22

MONTH: 08

YEAR: 2006

Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Keywords Mesh Terms:

KEYWORDS: Tissue Distribution

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism. Information

Substance Name: Proprotein Convertase 2

Registry Number: EC 3.4.21.94

Grant and Affiliation Information for Cellular colocalization and coregulation between hypothalamic pro-TRH and prohormone convertases in hypothyroidism.

AFFILIATION: Charles R. Drew Univ. of Medicine & Sciences, Division of Endocrinology, 1731 E. 120th St., Los Angeles, CA 90059, USA.

Country: United States

AGENCY: United States NCRR

GRANT: U54 RR-14616-01

ACRONYM: RR

MEDLINETA: Am J Physiol Endocrinol Metab

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