Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice.

Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Research Abstract Details 

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Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Abstract Text:

Kiyohiko Angata,Wenjau Lee,Junya Mitoma,Jamey D Marth,Minoru Fukuda,

Recent studies demonstrate that carbohydrates synthesized by specific glycosyltransferases play important roles in the development of the central nervous system. Among these carbohydrates, polysialic acid is a unique glycan that modulates functions of the neural cell adhesion molecule (NCAM) by attenuating NCAM-mediated interaction between neural cells. During brain development, polysialic acid is synthesized in a specific spatiotemporal pattern by two polysialyltransferases, ST8SiaII and ST8SiaIV. To study in vivo the roles of polysialic acid synthesized by each respective enzyme, we generated ST8SiaII and ST8SiaIV knockout mice. Single knockout ST8SiaII or ST8SiaIV mice show polysialic acid expression patterns differing from wild type, and those patterns indicate different roles of each gene during neural development. In this chapter, we discuss methods used to analyze polysialyltransferase knockout mice using immunohistochemistry of brain and primary cultures of neurons.

Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Publishing Authors By Initials

K Angata,W Lee,J Mitoma,JD Marth,M Fukuda,

For similar nervous system research abstracts see: nervous system research

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Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Journal Published:

PUBLICATION TYPE: Review

Journal: Methods in enzymology

VOLUME: 417

Page Numbers: 25-37

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2006

Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Information

Number of References: 33

LANGUAGE: eng

NlmUniqueID: 212271

Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Keywords Mesh Terms:

KEYWORDS: Nervous System

MESH TERMS: enzymology

Chemical & Substance for Abstract: Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Information

Substance Name: Glycosyltransferases

Registry Number: EC 2.4.-

Grant and Affiliation Information for Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice.

AFFILIATION: Glycobiology Program, Cancer Center, Burnham Institute for Medical Research, La Jolla, California, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK48247

ACRONYM: DK

MEDLINETA: Methods Enzymol

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