Cell type-specific DNA methylation patterns in the human breast.

Cell type-specific DNA methylation patterns in the human breast. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cell type-specific DNA methylation patterns in the human breast. Abstract Text:

Cellular identity and differentiation are determined by epigenetic programs. The characteristics of these programs in normal human mammary epithelium and their similarity to those in stem cells are unknown. To begin investigating these issues, we analyzed the DNA methylation and gene expression profiles of distinct subpopulations of mammary epithelial cells by using MSDK (methylation-specific digital karyotyping) and SAGE (serial analysis of gene expression). We identified discrete cell-type and differentiation state-specific DNA methylation and gene expression patterns that were maintained in a subset of breast carcinomas and correlated with clinically relevant tumor subtypes. CD44+ cells were the most hypomethylated and highly expressed several transcription factors with known stem cell function including HOXA10 and TCF3. Many of these genes were also hypomethylated in BMP4-treated compared with undifferentiated human embryonic stem (ES) cells that we analyzed by MSDK for comparison. Further highlighting the similarity of epigenetic programs of embryonic and mammary epithelial cells, genes highly expressed in CD44+ relative to more differentiated CD24+ cells were significantly enriched for Suz12 targets in ES cells. The expression of FOXC1, one of the transcription factors hypomethylated and highly expressed in CD44+ cells, induced a progenitor-like phenotype in differentiated mammary epithelial cells. These data suggest that epigenetically controlled transcription factors play a key role in regulating mammary epithelial cell phenotypes and imply similarities among epigenetic programs that define progenitor cell characteristics.

Cell type-specific DNA methylation patterns in the human breast. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cell type-specific DNA methylation patterns in the human breast. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 105

Page Numbers: 14076-81

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 9

MONTH: 09

YEAR: 2008

Cell type-specific DNA methylation patterns in the human breast. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Cell type-specific DNA methylation patterns in the human breast. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cell type-specific DNA methylation patterns in the human breast. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cell type-specific DNA methylation patterns in the human breast.

AFFILIATION: Departments of Medical Oncology and Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA 02115.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cell type-specific DNA methylation patterns in the human breast Related Publications

• Arbidol: A Broad-Spectrum Antiviral Compound that Blocks Viral Fusion.
• Artifactual detection of biotin on histones by streptavidin.
• Biochemical mechanism of hepatitis C virus inhibition by the broad-spectrum antiviral arbidol.
• Breast cancer stem cells: a case of mistaken identity?
• Breast cancer: origins and evolution.
• Breast tumor heterogeneity: cancer stem cells or clonal evolution?
• Cell type-specific DNA methylation patterns in the human breast.
• Epithelial and Stromal Cathepsin K and CXCL14 Expression in Breast Tumor Progression.
• FMC7 is an epitope of CD20.
• Is breast tumor progression really linear?
• Methylation-specific digital karyotyping.
• Microbial biotin protein ligases aid in understanding holocarboxylase synthetase deficiency.
• Microenvironmental regulation of cancer development.
• Reduced half-life of holocarboxylase synthetase from patients with severe multiple carboxylase deficiency.
• Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infection.
• Regulation of in situ to invasive breast carcinoma transition.
• The cancer stem cell hypothesis: in search of definitions, markers, and relevance.
• [Daily variation in intraocular pressure.]