Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens.

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Abstract Text:

Mei Hua Hong,Hong Sun,Cheng He Jin,Mark Chapman,Junlian Hu,William Chang,Kelven Burnett,Jon Rosen,Andres Negro-Vilar,Jeffrey N Miner,

Although it is evident that androgens increase muscle mass and strength, little is known about the critical molecular targets of androgens in skeletal muscle. In rodents, the skeletal alpha-actin gene is a tissue-specific gene expressed only in the levator ani and other skeletal muscles but not in the prostate or preputial gland, the well-known androgen target tissue. We identified tissue-specific androgen-regulated genes in the skeletal muscle in rats after oral administration of androgens and focused on androgen-dependent up-regulation of the skeletal alpha-actin gene. To investigate the mechanism of action, an in vitro system with various cell lines and a series of deletion mutants of the alpha-actin promoter were used. The human skeletal alpha-actin promoter was activated by androgens in the muscle cell line C2C12 but not in the liver, prostate, or breast cancer cell lines in which exogenous human androgen receptor is expressed. The sequence of the promoter is sufficient for cell-specific androgen response, providing a model for the tissue specificity demonstrated in vivo. Using a series of deletion mutants, the androgen response can be maintained using just the proximal promoter region. The importance of androgen regulation of this small portion of the human skeletal alpha-actin promoter was demonstrated by the correlation between muscle and the alpha-actin promoter activity for an array of selective androgen receptor modulators (SARMs), including an orally active SARM LGD2226. Taken together, the results suggest that the regulation of skeletal alpha-actin by androgens/SARMs may represent an important model system for understanding androgen anabolic action in the muscle.

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Publishing Authors By Initials

MH Hong,H Sun,CH Jin,M Chapman,J Hu,W Chang,K Burnett,J Rosen,A Negro-Vilar,JN Miner,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Endocrinology

VOLUME: 149

Page Numbers: 1103-12

Journal Abbreviation: Endocrinology

ISSN: 0013-7227

DAY: 6

MONTH: 12

YEAR: 2007

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375040

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens.

AFFILIATION: Department of Molecular and Cell Biology, Ligand Pharmaceuticals Inc., 10275 Science Center Drive, San Diego, California 92121. mhong@ligand.com.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Endocrinology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens Related Publications

• 15-Second conducted electrical weapon exposure does not cause core temperature elevation in non-environmentally stressed resting adults.
• Academic achievement and academic placement following traumatic brain injury in children and adolescents: a two-year longitudinal study.
• Cell-Specific Activation of the Human Skeletal {alpha}-Actin by Androgens.
• Comparing pain severity and functional status of older adults without spinal symptoms, with lumbar spinal stenosis, and with axial low back pain.
• Correlation of the International Prostate Symptom Score bother question with the Benign Prostatic Hyperplasia Impact Index in a clinical practice setting.
• Disruption of glomerular basement membrane charge through podocyte-specific mutation of agrin does not alter glomerular permselectivity.
• Evidence for physiological effects of hypercortisolemia in psychiatric patients.
• Formation within sensory nucleus V of synaptic associations mediating cutaneous localization.
• HIV Issues and Mapuches in Chile.
• In vivo comparison of hip mechanics for minimally invasive versus traditional total hip arthroplasty.
• Increased progerin expression associated with unusual LMNA mutations causes severe progeroid syndromes.
• Intravenous esomeprazole 40 mg vs. intravenous lansoprazole 30 mg for controlling intragastric acidity in healthy adults.
• Keratinocyte-specific expression of fatty acid transport protein 4 rescues the wrinkle-free phenotype in Slc27a4/Fatp4 mutant mice.
• LGD-5552, an antiinflammatory glucocorticoid receptor ligand with reduced side effects, in vivo.
• Laminins and their roles in mammals.
• Logic model use in developing a survey instrument for program evaluation: emergency preparedness summits for schools of nursing in Georgia.
• Partial rescue of glomerular laminin alpha5 mutations by wild-type endothelia produce hybrid glomeruli.
• Public health nursing and a provincial medical insurance plan.
• Public health nursing implications.
• SOCIAL HYGIENE AND VENEREAL DISEASE CONTROL.
• Sonographic diagnosis of ascending transtentorial herniation of posterior fossa cystic masses.
• Specificity of needle electromyography for lumbar radiculopathy and plexopathy in 55- to 79-year-old asymptomatic subjects.
• Surgical management of gastric cancer: review and consideration for total care of the gastric cancer patient.
• THE PUBLIC HEALTH NURSE IN DIAGNOSIS AND TREATMENT.
• The LG1-3 tandem of laminin alpha5 harbors the binding sites of Lutheran/basal cell adhesion molecule and alpha3beta1/alpha6beta1 integrins.
• The effects of stanozolol and boldenone undecylenate on scrotal width, testis weight, and sperm production in pony stallions.
• The efficacy of bovoflavin salve in the experimental treatment of trichomoniasis in bulls.
• The oath.
• Treatment, management, and monitoring of established rheumatoid arthritis.
• Verbal and nonverbal skill discrepancies in hydrocephalic children.