Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth.

Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Research Abstract Details 

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Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Abstract Text:

Audra Day,Jody Markwardt,Rolando Delaguila,Jian Zhang,Kedar Purnapatre,Saul M Honigberg,Brandt L Schneider,

In the yeast Saccharomyces cerevisiae, mitotic cell cycle progression depends upon the G(1)-phase cyclin-dependent kinase Cln-Cdc28 and cell growth to a minimum cell size. In contrast, Cln-Cdc28 inhibits entry into meiosis, and a cell growth requirement for sporulation has not been established. Here, we report that entry into meiosis also depends upon cell growth. Moreover, sporulation and cell growth rates were proportional to cell size; large cells grew rapidly and sporulated sooner while smaller cells grew slowly and sporulated later. In addition, Cln2 protein levels were higher in smaller cells suggesting that Cln-Cdc28 activity represses meiosis in smaller cells by preventing cell growth. In support of this hypothesis, loss of Clns, or the presence of a cdc28 mutation increased cell growth specifically in smaller cells and accelerated meiosis in these cells. Finally, overexpression of CLNs repressed meiosis in smaller cells, but not in large cells. Taken together, these results demonstrate that Cln-Cdc28 represses entry into meiosis in part by inhibiting cell growth.

Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Publishing Authors By Initials

A Day,J Markwardt,R Delaguila,J Zhang,K Purnapatre,SM Honigberg,BL Schneider,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Cell cycle (Georgetown, Tex.)

VOLUME: 3

Page Numbers: 1433-9

Journal Abbreviation: Cell Cycle

ISSN: 1551-4005

DAY: 6

MONTH: 11

YEAR: 2004

Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101137841

Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth. Information

Substance Name: CDC28 Protein Kinase, S cerevisiae

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Cell size and Cln-Cdc28 complexes mediate entry into meiosis by modulating cell growth.

AFFILIATION: Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.

Country: United States

AGENCY: United States NICHD

GRANT: T32HD07271-19

ACRONYM: HD

MEDLINETA: Cell Cycle

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