Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence.

Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence. Research Abstract Details 

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Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence. Abstract Text:

Wan Long Zhu,Yong Hai Nan,Kyung-Soo Hahm,Song Yub Shin,Wan Long Zhu,Yong Hai Nan,Kyung-Soo Hahm,Song Yub Shin,Wan Long Zhu,Yong Hai Nan,Kyung-Soo Hahm,Song Yub Shin,

Melittin (ME), a linear 26-residue non-cell-selective antimicrobial peptide, displays strong lytic activity against bacterial and human red blood cells. To design ME analogue with improved cell selectivity, we synthesized a melittin diastereomer (ME-D) with D-amino acid in the leucine zipper sequence (Leu-6, Lue-13 and Ile-20). Compared to ME, ME-D exhibited the same or 2-fold higher antibacterial activity but 8-fold less hemolytic activity. Circular dichroism analysis revealed that ME-D has much less alpha-helical content in alpha-helical content in the presence of zwitterionic EYPC/cholesterol (10 : 1, w/w) liposomes compared to negatively charged EYPE/EYPG (7 : 3, w/w) liposomes. The blue shift of the fluorescence emission maximum of ME-D in zwitterionic EYPC/ cholesterol (10 : 1, w/w) liposomes was much smaller than in negatively charged EYPE/EYPG (7 : 3, w/w) liposomes. These results suggested that the improvement in therapeutic index/cell selectivity of ME-D is correlated with its less permeability to zwitterionic membranes.

Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence. Publishing Authors By Initials

WL Zhu,YH Nan,KS Hahm,SY Shin,WL Zhu,YH Nan,KS Hahm,SY Shin,WL Zhu,YH Nan,KS Hahm,SY Shin,

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Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry and molecular biology

VOLUME: 40

Page Numbers: 1090-4

Journal Abbreviation: J. Biochem. Mol. Biol.

ISSN: 1225-8687

DAY: 30

MONTH: Nov

YEAR: 2007

Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence. Information

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LANGUAGE: eng

NlmUniqueID: 9702084

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Grant and Affiliation Information for Cell Selectivity of an Antimicrobial Peptide Melittin Diastereomer with D-amino Acid in the Leucine Zipper Sequence.

AFFILIATION: Department of Bio-Materials, Graduate School, Chosun University, Gwangju 501-759, Korea syshin@chosun.ac.kr.

Country: Korea (South)

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MEDLINETA: J Biochem Mol Biol

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