Cell labeling with the positive MR contrast agent Gadofluorine M.

Cell labeling with the positive MR contrast agent Gadofluorine M. Research Abstract Details 

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Cell labeling with the positive MR contrast agent Gadofluorine M. Abstract Text:

Tobias D Henning,Olaf Saborowski,Daniel Golovko,Sophie Boddington,Jan S Bauer,Yanjun Fu,Reinhard Meier,Hubertus Pietsch,Barbara Sennino,Donald M McDonald,Heike E Daldrup-Link,

The purpose of this study was to label human monocytes with Gadofluorine M by simple incubation for subsequent cell depiction at 1.5 and 3 T. Gadofluorine M displays a high r(1) relaxivity and is spontaneously phagocytosed by macrophages. Human monocytes were incubated with Gadofluorine M-Cy at varying concentrations and incubation times and underwent MR imaging at 1.5 and 3 T at increasing time intervals after the labeling procedure. R1-relaxation rates and r1 relaxivities of the labeled cells and non-labeled controls were determined. Cellular contrast agent uptake was examined by fluorescence microscopy and quantified by ICP-AES. Efficient cell labeling was achieved after incubation of the cells with 25 mM Gd Gadofluorine M for 12 h, resulting in a maximal uptake of 0.3 fmol Gd/cell without impairment of cell viability. Fluorescence microscopy confirmed internalization of the fluorescent contrast agent by monocytes. The r1 relaxivity of the labeled cells was 137 mM(-1)s(-1) at 1.5 T and 80.46 mM(-1)s(-1) at 3 T. Imaging studies showed stable labeling for at least 7 days. Human monocytes can be effectively labeled for MR imaging with Gadofluorine M. Potential in vivo cell-tracking applications include targeting of inflammatory processes with Gadofluorine-labeled leukocytes or monitoring of stem cell therapies for the treatment of arthritis.

Cell labeling with the positive MR contrast agent Gadofluorine M. Publishing Authors By Initials

TD Henning,O Saborowski,D Golovko,S Boddington,JS Bauer,Y Fu,R Meier,H Pietsch,B Sennino,DM McDonald,HE Daldrup-Link,

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Cell labeling with the positive MR contrast agent Gadofluorine M. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European radiology

VOLUME: 17

Page Numbers: 1226-34

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ISSN: 0938-7994

DAY: 6

MONTH: 01

YEAR: 2007

Cell labeling with the positive MR contrast agent Gadofluorine M. Information

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LANGUAGE: eng

NlmUniqueID: 9114774

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Grant and Affiliation Information for Cell labeling with the positive MR contrast agent Gadofluorine M.

AFFILIATION: Department of Radiology, University of California in San Francisco, 505 Parnassus Ave, San Francisco, CA, 94143, USA, daldrup@radiology.ucsf.edu.

Country: Germany

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MEDLINETA: Eur Radiol

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