Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria.

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Abstract Text:

Y Yoshida,T Hashimoto,K Tagawa,

ATPase inhibitor protein, which blocks mitochondrial ATPase activity by forming an enzyme-inhibitor complex, was found to be synthesized as a larger precursor in a cell-free translation system directed by yeast mRNA. Other protein factors, which stabilize latent ATPase by binding to the enzyme-inhibitor complex, were also found to be formed as larger precursors. The precursor of ATPase inhibitor protein was transported into isolated yeast mitochondria and was cleaved to the mature peptide in the mitochondria. Impaired mitochondria lacking phosphorylation activity could not convert the precursor to the mature form. Neither antimycin A nor oligomycin alone exhibited a marked effect on the transport-processing of the precursor by intact mitochondria. However, when antimycin A was added with oligomycin, the transport-processing was markedly inhibited. The processing was also strongly inhibited by an uncoupler, carbonylcyanide p-trifluoro-methoxyphenyl hydrazone. The inhibition by the uncoupler was not relieved by ATP added externally. It is concluded that the transport-processing of precursor proteins requires intact mitochondria with a potential difference across the inner membrane.

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Publishing Authors By Initials

Y Yoshida,T Hashimoto,K Tagawa,

For similar fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research abstracts see: fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research

PUBMED ID PMID:

MEDLINE DATE:

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 283-90

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1983

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria Related Publications

• 8-Epi-iridodial glucoside from Boschniakia rossica.
• A direct colorimetric method for the determination of phospholipids with dithiocyanatoiron reagent.
• A simple method for measuring phosphatidylcholine as its hydrophobic complex with tetrathiocyanatocobaltate.
• A stabilizing factor of yeast mitochondrial F1F0-ATPase-inhibitor complex: common amino acid sequence with yeast ATPase inhibitor and E. coli epsilon and bovine delta subunits.
• Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from yeast.
• An immuno-light- and electron-microscopic study of the expression of bone morphogenetic protein-2 during the process of ectopic bone formation in the rat.
• Binding of intrinsic ATPase inhibitor to mitochondrial ATPase--stoichiometry of binding of nucleotides, inhibitor, and enzyme.
• Binding properties of 9K protein to F1-ATPase: a counterpart ligand to the ATPase inhibitor.
• Binding properties of an intrinsic ATPase inhibitor and occurrence in yeast mitochondria of a protein factor which stabilizes and facilitates the binding of the inhibitor to F1F0-ATPase.
• Botulinum toxin treatment for blepharospasm associated with myasthenia gravis.
• Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria.
• Change in P-450 content accompanying aerobic formation of mitochondria in yeast.
• Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study.
• Decrease in mitochondrial levels of adenine nucleotides and concomitant mitochondrial dysfunction in ischemic rat liver.
• Development of mitochondrial membranes in anaerobically grown yeast cells.
• Energy-independent protection of the oxidative phosphorylation capacity of mitochondria against anoxic damage by ATP and its non-metabolizable analogs.
• Existence of stoichiometric amounts of an intrinsic ATPase inhibitor and two stabilizing factors with mitochondrial ATP synthase in yeast.
• Hepatic accumulation of pyrophosphate during acetate metabolism.
• Interaction of mitochondrial aspartate aminotransferase with negatively charged lecithin liposomes.
• Involvement of Ca2+ release and activation of phospholipase A2 in mitochondrial dysfunction during anoxia.
• Kinetics of hydroperoxide degradation by NADP-glutathione system in mitochondria.
• Localization of cytochrome c peroxidase in anaerobically grown yeast cells.
• Non-mitochondrial electron transfer system in anaerobically grown yeast cells.
• Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography.
• Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria.
• Purification and properties of factors in yeast mitochondria stabilizing the F1F0-ATPase-inhibitor complex.
• Simultaneous bindings of ATPase inhibitor and 9K protein to F1F0-ATPase in the presence of 15K protein in yeast mitochondria.
• [Hypertension in dialysis patients]
• [Hypotension in dialysis patients]
• pH-Induced conformational change of ATPase inhibitor from yeast mitochondria. A proton magnetic resonance study.