Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA.

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Abstract Text:

A full-length cloned cDNA insert containing the sequence of potato spindle tuber viroid (PSTV) was dimerized and placed in the plasmid vector pSP65 adjacent to a promoter for bacteriophage SP6 RNA polymerase. In vitro transcription of this region yielded a single linear RNA chain 760 bases in length containing two copies of PSTV RNA with about 20 bases of vector sequence at each end. Bioassay on tomato plants revealed that this transcript has infectivity comparable to that of PSTV itself, yielding circular progeny RNAs indistinguishable from PSTV grown in vivo Comparative RNA fingerprinting analysis revealed that the viroid sequence in the dimeric transcript breeds true (compared to control viroid strains of different sequence grown in parallel) but loses the vector-specific sequences during growth in plants. Incubation of (32)P-labeled dimeric PSTV RNA at neutral pH and 39 degrees gave a 1-5% yield of three RNA segments, one comigrating with unit-length linear PSTV and two smaller fragments. Reaction of the unit-length cleavage product with wheat germ RNA ligase gave a high yield of circular molecules indistinguishable from PSTV circles arising in vivo, suggesting that the cleavage reaction yields 2',3' cyclic phosphate termini and could represent a part of the in vivo PSTV replication cycle.

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Virology

VOLUME: 142

Page Numbers: 441-7

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 30

MONTH: Apr

YEAR: 1985

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 110674

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA.

AFFILIATION: The Rockefeller University, New York, New York 10021, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Virology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA Related Publications

• A review of the topical use of antibiotics.
• Bacillus Calmette-Guérin (B.C.G.): Animal Experimentation and Prophylactic Immunization of Children.
• Cell-free synthesis and processing of an infectious dimeric transcript of potato spindle tuber viroid RNA.
• Cellular therapies: a Canadian blood services research and development symposium.
• Creatine supplementation and exercise performance: an update.
• Description of the male of Cosmolaelaps gurabensis Fox (Acarina, Laelaptidae).
• Differences in the behavioral time course of effects of rate-increasing and rate-decreasing doses of cocaine in pigeons.
• Direct assessment of ADL in Alzheimer's disease.
• Empirical validation of a procedure to correct position and stimulus biases in matching-to-sample.
• Evaluation of flurbiprofen urinary ratios as in vivo indices for CYP2C9 activity.
• Evidence-based medicine: raising the bar.
• How Oklahoma fights cancer.
• Influence of E.C.T. and insulin coma on level of adrenocortical steroids in peripheral circulation.
• Inheritance of a one-seeded pod trait in peanut.
• Is Rorty's Neopragmatism the "Real" Foundation of Medical Ethics: a Search for Foundational Principles.
• Laser-Induced Fluorescence Excitation Spectroscopy of the Magnesium Oxide B(1)?(+)-A(1)nu system.
• Liver Fibrosis during an Outbreak of Acute Hepatitis C Virus Infection in HIV-Infected Men: A Prospective Cohort Study.
• Meningeal sarcoma with extracranial metastasis.
• Molecular and bioinformatic evidence of hepatitis C virus evolution in brain.
• Outpatient termination of pregnancy.
• Predictors of hospital mortality in older patients with subdural hematoma.
• Prevalence and risk of Plasmodium falciparum and P. vivax malaria among pregnant women living in the hypoendemic communities of the Peruvian Amazon.
• Risk behaviour, healthcare access and prevalence of infection with Chlamydia trachomatis and Neisseria gonorrhoeae in a population-based sample of adults in Barbados.
• Role of endoscopic retrograde cholangiopancreatography in acute pancreatitis.
• THE GRADATIONAL STEP TEST FOR ASSESSING CARDIORESPIRATORY CAPACITY: AN EXPERIMENTAL EVALUATION OF TREADMILL AND STEP TEST PROCEDURES.
• The long acting sulfonamide drugs.
• The snake family Psammophiidae (Reptilia: Serpentes): Phylogenetics and species delimitation in the African sand snakes (Psammophis Boie, 1825) and allied genera.
• Unilaterally nephrectomized rat with aortic ligation: a uremic model of severe hypertension.
• Wheelchair users are not necessarily wheelchair bound.
• Within-session patterns in variable-interval schedule performance: Variation with deprivation level.