Cell death in an ischemic infarct rim model.

Cell death in an ischemic infarct rim model. Research Abstract Details 

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Cell death in an ischemic infarct rim model. Abstract Text:

Hang Yao,Xiaolu Sun,Xiang Gu,Juan Wang,Gabriel G Haddad,Hang Yao,Xiaolu Sun,Xiang Gu,Juan Wang,Gabriel G Haddad,

Using an in vitro model that simulates the microenvironment in the ischemic infarct rim, we have examined the temporal profile and possible mechanisms of cell death in the neuropil (an astrocyte-rich area or ARA) of organotypic hippocampal slice cultures. Two-photon confocal microscopy, propidium iodide, and GFAP-GFP transgenic mice were used to confirm cell death in astrocytes. An 'ischemic solution' (IS) induced major cell death throughout the hippocampus over 24 h, with the earliest injury starting in ARA. Our studies using IS or ion replacements in IS revealed that cell death in ARA was modest when K(+) was increased or pH lowered. High K(+) is most effective in reducing cell death when HCO(3)(-) is normal or high. When Cl(-) or HCO(3)(-) was reduced, cell injury was worsened. 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) protected cells from IS-induced death in a dose-dependent manner (1-4000 micromol/L). We conclude that (i) various areas of the hippocampal formation respond differently to ionic replacements; (ii) K(+) interacts with other ions to protect cells in ARA; and (iii) DIDS has a substantial protective effect in ARA by blocking DIDS-sensitive membrane exchangers or by interfering with intracellular signaling pathways.

Cell death in an ischemic infarct rim model. Publishing Authors By Initials

H Yao,X Sun,X Gu,J Wang,GG Haddad,H Yao,X Sun,X Gu,J Wang,GG Haddad,

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Cell death in an ischemic infarct rim model. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neurochemistry

VOLUME: 103

Page Numbers: 1644-53

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 28

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 2985190

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Grant and Affiliation Information for Cell death in an ischemic infarct rim model.

AFFILIATION: Department of Pediatrics (Section of Respiratory Medicine), University of California, San Diego, La Jolla, California, USA.

Country: England

AGENCY: United States NHLBI

GRANT: R01 HL 66237

ACRONYM: HL

MEDLINETA: J Neurochem

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