Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum.

Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Research Abstract Details 

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Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Abstract Text:

S Goodenough,M Davidson,G Kidd,I Matsumoto,P Wilce,

Administration of polyamines into the central nervous system results in tissue damage, possibly through the excitotoxic actions of the NMDA receptor. Direct injection of 100 nmol of spermine into the rat striatum produced a lesion equivalent to approximately 50% of the striatum. Analysis of the DNA in this region revealed the distinct ladder-like pattern of degradation often associated with apoptosis. This DNA fragmentation was confirmed in vivo using terminal deoxynucleotidyl-transferase-mediated biotinylated deoxyuridine triphosphate nick end labelling (TUNEL). The morphology of the TUNEL-positive cells showed marked differences at the needle tract when compared with cells in damaged areas away from the needle tract, suggesting a differential mechanism of cell death in these two regions. The patterns of p53, c-Fos and c-Jun protein expression were determined using immunohistochemistry. The number of p53-immunoreactive cells increased up to 14 h and returned to basal levels by 24 h. c-Fos protein expression transiently increased, peaking at 8 h after injection. c-Jun exhibited a protracted pattern of expression, remaining elevated up to 24 h. p53 protein expression was colocalised with TUNEL staining in areas away from the needle tract, but not in cells at the needle tract, suggesting once again a differential mechanism of cell death. At 14 h, c-Fos and c-Jun were not colocalised with TUNEL staining, suggesting that they are either not involved with the cell death process or that the time course of protein expression and the onset of DNA fragmentation do not overlap. This work represents the first characterisation of processes associated with cell death induced by spermine in vivo.

Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Publishing Authors By Initials

S Goodenough,M Davidson,G Kidd,I Matsumoto,P Wilce,

For similar proteins: dna-binding proteins: tumor suppressor protein p53 research abstracts see: proteins: dna-binding proteins: tumor suppressor protein p53 research

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Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental brain research. Experimentelle Hirnfo

VOLUME: 131

Page Numbers: 126-34

Journal Abbreviation:

ISSN: 0014-4819

DAY: 15

MONTH: Mar

YEAR: 2000

Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Information

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LANGUAGE: eng

NlmUniqueID: 43312

Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Protein p53

MESH TERMS: analysis

Chemical & Substance for Abstract: Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum. Information

Substance Name: Spermine

Registry Number: 71-44-3

Grant and Affiliation Information for Cell death and immunohistochemistry of p53, c-Fos and c-Jun after spermine injection into the rat striatum.

AFFILIATION: Department of Biochemistry, The University of Queensland, St Lucia, Australia.

Country: GERMANY

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MEDLINETA: Exp Brain Res

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