Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias.

Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Research Abstract Details 

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Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Abstract Text:

Angela Parton,David Forest,Hiroshi Kobayashi,Lori Dowell,Christopher Bayne,David Barnes,

Cartilaginous fish, primarily sharks, rays and skates (elasmobranchs), appeared 450 million years ago. They are the most primitive vertebrates, exhibiting jaws and teeth, adaptive immunity, a pressurized circulatory system, thymus, spleen, and a liver comparable to that of humans. The most used elasmobranch in biomedical research is the spiny dogfish shark, Squalus acanthias. Comparative genomic analysis of the dogfish shark, the little skate (Leucoraja erincea), and other elasmobranchs have yielded insights into conserved functional domains of genes associated with human liver function, multidrug resistance, cystic fibrosis, and other biomedically relevant processes. While genomic information from these animals is informative in an evolutionary framework, experimental verification of functions of genomic sequences depends heavily on cell culture approaches. We have derived the first multipassage, continuously proliferating cell line of a cartilaginous fish. The line was initiated from embryos of the spiny dogfish shark. The cells were maintained in a medium modified for fish species and supplemented with cell type-specific hormones, other proteins and sera, and plated on a collagen substrate. SAE cells have been cultured continuously for three years. These cells can be transfected by plasmids and have been cryopreserved. Expressed Sequence Tags generated from a normalized SAE cDNA library included a number of markers for cartilage and muscle, as well as proteins influencing tissue differentiation and development, suggesting that SAE cells may be of mesenchymal stem cell origin. Examination of SAE EST sequences also revealed a cartilaginous fish-specific repetitive sequence that may be evidence of an ancient mobile genetic element that most likely was introduced into the cartilaginous fish lineage after divergence from the lineage leading to teleosts.

Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Publishing Authors By Initials

A Parton,D Forest,H Kobayashi,L Dowell,C Bayne,D Barnes,

For similar animals: chordata: vertebrates: fishes: elasmobranchii: sharks: dogfish: squalus research abstracts see: animals: chordata: vertebrates: fishes: elasmobranchii: sharks: dogfish: squalus research

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Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Comparative biochemistry and physiology. Toxicolog

VOLUME: 145

Page Numbers: 111-9

Journal Abbreviation: Comp. Biochem. Physiol. C Toxi

ISSN: 1532-0456

DAY: 14

MONTH: 07

YEAR: 2006

Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100959500

Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Keywords Mesh Terms:

KEYWORDS: Squalus

MESH TERMS: physiology

Chemical & Substance for Abstract: Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Cell and molecular biology of SAE, a cell line from the spiny dogfish shark, Squalus acanthias.

AFFILIATION: Mount Desert Island Biological Laboratories, Salisbury Cove, ME 04672, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR016463

ACRONYM: RR

MEDLINETA: Comp Biochem Physiol C Toxicol

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