cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta.

cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta. Research Abstract Details 

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cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta. Abstract Text:

Juan Lin,Xuanwei Zhou,Shi Gao,Xiaojun Liu,Weisheng Wu,Xiaofen Sun,Kexuan Tang,

Pinellia pedatisecta agglutinin (PPA)is a very basic protein that accumulates in the tuber of P.pedatisecta .PPA is a hetero-tetramer protein of 40 kDa,composed of two polypeptide chains A (about 12 kDa)and two polypeptides chains B (about 12 kDa).The full-length cDNA of PPA was cloned from P.pedatisecta using SMART RACE-PCR technology; it was 1146 bp and contained a 771 bp open reading frame (ORF)encoding a lectin precursor of 256 amino acid residues with a 24 amino acid signal peptide.The PPA precursor contained 3 mannose-binding sites (QXDXNXVXY) and two conserved domains of 43% identity,PPA-DOM 1 (polypeptides A)and PPA-DOM 2 (polypeptides B).PPA shared varying identities,ranging from 40% to 85%,with mannose-binding lectins from other species of plant families such as Araceae,Alliaceae,Iridaceae,Liliaceae,Amaryllidaceae and Bromeliaceae.Southern blot analysis indicated that ppa belonged to a multi-copy gene family.Expression pattern analysis revealed that ppa expressed in most tested tissues,with high expression being found in spadix,spathe and tuber.Cloning of the ppa gene not only provides a basis for further investigation of its structure,expression and regulatory mechanism,but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into plants in the future.

cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta. Publishing Authors By Initials

J Lin,X Zhou,S Gao,X Liu,W Wu,X Sun,K Tang,

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cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biosciences

VOLUME: 32

Page Numbers: 241-9

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ISSN: 0250-5991

DAY: 16

MONTH: Mar

YEAR: 2007

cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta. Information

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LANGUAGE: eng

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Grant and Affiliation Information for cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta.

AFFILIATION: State Key Laboratory of Genetic Engineering,Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences,Morgan-Tan International Center for Life Sciences,Fudan University,Shanghai 200433,People's Republic of China.

Country: India

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MEDLINETA: J Biosci

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