CD99 is a key mediator of the transendothelial migration of neutrophils.

CD99 is a key mediator of the transendothelial migration of neutrophils. Research Abstract Details 

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CD99 is a key mediator of the transendothelial migration of neutrophils. Abstract Text:

Olivia Lou,Pilar Alcaide,Francis W Luscinskas,William A Muller,

Transendothelial migration of leukocytes is a critical event for inflammation, but the molecular regulation of this event is only beginning to be understood. PECAM (CD31) is a major mediator of monocyte and neutrophil transmigration, and CD99 was recently defined as a second mediator of the transmigration of monocytes. Expression of CD99 on the surface of circulating polymorphonuclear cells (PMN) is low compared with expression of CD99 on monocytes or expression of PECAM on PMN. We demonstrate here that, despite low expression of CD99, Fab of Abs against CD99 blocked over 80% of human neutrophils from transmigrating across HUVEC monolayers in an in vitro model of inflammation. Blocking CD99 on either the neutrophil or endothelial cell side resulted in a quantitatively equivalent block, suggesting a homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell. Blocking CD99 and PECAM together resulted in additive effects, suggesting the two molecules work at distinct steps. Confocal microscopy confirmed that CD99-blocked neutrophils lodged in endothelial cell junctions at locations distal to PECAM-blocked neutrophils. The CD99-blocked PMN exhibited dynamic lateral movement within endothelial cell junctions, indicating that only the diapedesis step was blocked by interference with CD99. Anti-CD99 mAb also blocked PMN transmigration in a second in vitro model that incorporated shear stress. Taken together, the evidence demonstrates that PECAM and CD99 regulate distinct, sequential steps in the transendothelial migration of neutrophils during inflammation.

CD99 is a key mediator of the transendothelial migration of neutrophils. Publishing Authors By Initials

O Lou,P Alcaide,FW Luscinskas,WA Muller,

For similar cells: blood cells: leukocytes: granulocytes: neutrophils research abstracts see: cells: blood cells: leukocytes: granulocytes: neutrophils research

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CD99 is a key mediator of the transendothelial migration of neutrophils. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 178

Page Numbers: 1136-43

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 15

MONTH: Jan

YEAR: 2007

CD99 is a key mediator of the transendothelial migration of neutrophils. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

CD99 is a key mediator of the transendothelial migration of neutrophils. Keywords Mesh Terms:

KEYWORDS: Neutrophils

MESH TERMS: metabolism

Chemical & Substance for Abstract: CD99 is a key mediator of the transendothelial migration of neutrophils. Information

Substance Name: CD99 protein, human

Registry Number: 126466-52-2

Grant and Affiliation Information for CD99 is a key mediator of the transendothelial migration of neutrophils.

AFFILIATION: Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 53993

ACRONYM: HL

MEDLINETA: J Immunol

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