CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response.

CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response. Research Abstract Details 

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CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response. Abstract Text:

C ,N Roher,J C Balasch,L Ribas,F W Goetz,J V Planas,L Tort,S MacKenzie,

CD83, a cell surface membrane glycoprotein member of the Ig superfamily which is commonly used as standard surface marker for dendritic cells, was cloned from gilthead sea bream macrophages using degenerate primers against conserved motifs of known CD83 sequences. The obtained cDNA contains an open reading frame of 669 nucleotides that translate into a 222 amino acid putative peptide. The deduced protein sequence shows conservation of features shared by vertebrate CD83 and multiple alignment with fish CD83 sequences reveals high homology. In cultured sea bream macrophages CD83 mRNA expression was significantly enhanced in a dose- and time-dependent fashion after stimulation with Escherichia coli LPS. These results indicate that in fish, macrophages express high levels of CD83 mRNA after LPS exposure and CD83 is therefore a good marker for activated mature myeloid cells in fish.

CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response. Publishing Authors By Initials

C ,N Roher,JC Balasch,L Ribas,FW Goetz,JV Planas,L Tort,S MacKenzie,

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CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Fish & shellfish immunology

VOLUME: 23

Page Numbers: 877-85

Journal Abbreviation: Fish Shellfish Immunol.

ISSN: 1050-4648

DAY: 6

MONTH: 04

YEAR: 2007

CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response. Information

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LANGUAGE: eng

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AFFILIATION: Unitat de Fisiología Animal, Departament de Biologia Cellular, Fisiología i d'Immunologia, Facultat de Ciencies, Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain.

Country: England

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MEDLINETA: Fish Shellfish Immunol

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