CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1.

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Abstract Text:

Jeff S Isenberg,Lisa A Ridnour,Julie Dimitry,William A Frazier,David A Wink,David D Roberts,

CD36 is necessary for inhibition of some angiogenic responses by the matricellular glycoprotein thrombospondin-1 and is therefore assumed to be the receptor that mediates its anti-angiogenic activities. Although ligation of CD36 by antibodies, recombinant type 1 repeats of thrombospondin-1, or CD36-binding peptides was sufficient to inhibit nitric oxide (NO)-stimulated responses in both endothelial and vascular smooth muscle cells, picomolar concentrations of native thrombospondin-1 similarly inhibited NO signaling in vascular cells from wild-type and CD36-null mice. Ligation of the thrombospondin-1 receptor CD47 by recombinant C-terminal regions of thrombospondin-1, thrombospondin-1 peptides, or CD47 antibodies was also sufficient to inhibit NO-stimulated phenotypic responses and cGMP signaling in vascular cells. Thrombospondin-1 did not inhibit NO signaling in CD47-null vascular cells or NO-stimulated vascular outgrowth from CD47-null muscle explants in three-dimensional cultures. Furthermore, the CD36-binding domain of thrombospondin-1 and anti-angiogenic peptides derived from this domain failed to inhibit NO signaling in CD47-null cells. Therefore, ligation of either CD36 or CD47 is sufficient to inhibit NO-stimulated vascular cell responses and cGMP signaling, but only CD47 is necessary for this activity of thrombospondin-1 at physiological concentrations.

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Publishing Authors By Initials

JS Isenberg,LA Ridnour,J Dimitry,WA Frazier,DA Wink,DD Roberts,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 26069-80

Journal Abbreviation:

ISSN: 0021-9258

DAY: 11

MONTH: 07

YEAR: 2006

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1.

AFFILIATION: Laboratory of Pathology, National Institutes of Health, Bethesda, Maryland 20892, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1 Related Publications

• A pilot trial of plasma infusions in Duchenne muscular dystrophy.
• Acute effect of vasectomy on the function of the rat epididymal epithelium and vas deferens.
• Application of colorimetric assays to assess viability, growth and metabolism of hydrogel-encapsulated cells.
• CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1.
• Comparison of the antioxidant properties of alpha-tocopherol and alpha-tocopheryl acetate in newborn rabbit lung.
• Conceptus development, uterine response, blood gases and endocrine function of gilts exposed to increased ambient temperature during early pregnancy.
• Cost-effectiveness of low-molecular-weight heparin for secondary prophylaxis of cancer-related venous thromboembolism.
• Data-guided brain deformation modeling: evaluation of a 3-D adjoint inversion method in porcine studies.
• Deficiencies of cardiovascular risk prediction models for type 1 diabetes.
• Dementia and low testosterone and bioavailable testosterone levels in men: possible increased risk.
• Development of a real time quantitative PCR assay for the hard clam pathogen Quahog Parasite Unknown (QPX).
• Differential regulation of hepatic cytochrome P450 monooxygenases in streptozotocin-induced diabetic rats.
• Disposition of intravenously administered pharmacologic doses of vitamin E in newborn rabbits.
• Ethnic differences in adolescents' mental distress, social stress, and resources.
• Facts and ideas from anywhere.
• IRVING DAVID PRENGLER, MD, MBA: a conversation with the editor.
• Increasing the efficiency of Monte Carlo cohort simulations with variance reduction techniques.
• Origin and evolution of the TKDP gene family.
• PAUL BERNARD CONVERY, MD, MMM: a conversation with the editor.
• ROBERT GARY MENNEL, MD: a conversation with the editor.
• Relationship between doses of prostaglandin F(2alpha) and stages of the breeding season for synchronization of estrus and ovulation in ewes.
• Seasonal variation in gestational blood pressure.
• Sonic tooth brushing reduces gingival overgrowth in renal transplant recipients.
• Susceptibility of Escherichia coli to L-selenaproline and other L-proline analogues in laboratory culture media and normal human urine.
• Teaching medical decision modeling: a qualitative description of student errors and curriculum responses.
• The effect of age and role information on expectations for big five personality traits.
• The evolution of the art and science of theriogenology The Second David E. Bartlett Honorary Address.
• The occurrence of the cold-water coral Lophelia pertusa (Scleractinia) on oil and gas platforms in the North Sea: colony growth, recruitment and environmental controls on distribution.
• The p110alpha isoform of PI3K is essential for proper growth factor signaling and oncogenic transformation.
• Tissue vitamin E levels in newborn rabbits after pharmacologic dosing. Influence of dose, dosage form, and route of administration.