Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking.

Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking. Research Abstract Details 

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Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking. Abstract Text:

Zhiwei Liu,Hong Li,Manoocher Soleimani,Kuppuswami Girijashanker,Jodie M Reed,Lei He,Timothy P Dalton,Daniel W Nebert,Zhiwei Liu,Hong Li,Manoocher Soleimani,Kuppuswami Girijashanker,Jodie M Reed,Lei He,Timothy P Dalton,Daniel W Nebert,

The mouse Slc39a8 gene encodes the ZIP8 transporter, which has been shown to be a divalent cation/HCO3- symporter. Using ZIP8 cRNA-injected Xenopus oocyte cultures, we show herein that: [a] ZIP8-mediated cadmium (Cd(2+)) and zinc (Zn(2+)) uptake have V(max) values of 1.8+/-0.08 and 1.0+/-0.08 pmol/oocyte/h, and K(m) values of 0.48+/-0.08 and 0.26+/-0.09 microM, respectively; [b] ZIP8-mediated Cd(2+) uptake is most inhibited by Zn(2+), second-best inhibited by Cu(2+), Pb(2+) and Hg(2+), and not inhibited by Mn(2+) or Fe(2+); and [c] electrogenicity studies demonstrate an influx of two HCO3- anions per one Cd(2+) (or one Zn(2+)) cation, i.e. electroneutral complexes. Using Madin-Darby canine kidney (MDCK) polarized epithelial cells retrovirally infected with ZIP8 cDNA and tagged with hemagglutinin at the C-terminus, we show that-similar to ZIP4-the ZIP8 eight-transmembrane protein is largely internalized during Zn(2+) homeostasis, but moves predominantly to the cell surface membrane (trafficking) under conditions of Zn(2+) depletion.

Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking. Publishing Authors By Initials

Z Liu,H Li,M Soleimani,K Girijashanker,JM Reed,L He,TP Dalton,DW Nebert,Z Liu,H Li,M Soleimani,K Girijashanker,JM Reed,L He,TP Dalton,DW Nebert,

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Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemical and biophysical research communication

VOLUME: 365

Page Numbers: 814-20

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 26

MONTH: 11

YEAR: 2007

Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Cd2+ versus Zn2+ uptake by the ZIP8 HCO3--dependent symporter: kinetics, electrogenicity and trafficking.

AFFILIATION: Department of Environmental Health and Center for Environmental Genetics (CEG), University of Cincinnati Medical Center, P.O. Box 670056, Cincinnati, OH 45267-0056, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: R01 ES010416

ACRONYM: ES

MEDLINETA: Biochem Biophys Res Commun

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