CD1d-restricted NKT cells: an interstrain comparison.

CD1d-restricted NKT cells: an interstrain comparison. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

CD1d-restricted NKT cells: an interstrain comparison. Abstract Text:

CD1d-restricted Valpha14-Jalpha281 invariant alphabetaTCR(+) (NKT) cells are well defined in the C57BL/6 mouse strain, but they remain poorly characterized in non-NK1.1-expressing strains. Surrogate markers for NKT cells such as alphabetaTCR(+)CD4(-)CD8(-) and DX5(+)CD3(+) have been used in many studies, although their effectiveness in defining this lineage remains to be verified. Here, we compare NKT cells among C57BL/6, NK1.1-congenic BALB/c, and NK1.1-congenic nonobese diabetic mice. NKT cells were identified and compared using a range of approaches: NK1.1 expression, surrogate phenotypes used in previous studies, labeling with CD1d/alpha-galactosylceramide tetramers, and cytokine production. Our results demonstrate that NKT cells and their CD4/CD8-defined subsets are present in all three strains, and confirm that nonobese diabetic mice have a numerical and functional deficiency in these cells. We also highlight the hazards of using surrogate phenotypes, none of which accurately identify NKT cells, and one in particular (DX5(+)CD3(+)) actually excludes these cells. Finally, our results support the concept that NK1.1 expression may not be an ideal marker for CD1d-restricted NKT cells, many of which are NK1.1-negative, especially within the CD4(+) subset and particularly in NK1.1-congenic BALB/c mice.

CD1d-restricted NKT cells: an interstrain comparison. Publishing Authors By Initials

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: lymphocyte subsets: t-lymphocyte subsets research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: lymphocyte subsets: t-lymphocyte subsets research

PUBMED ID PMID:

MEDLINE DATE:

CD1d-restricted NKT cells: an interstrain comparison. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 167

Page Numbers: 1164-73

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 1

MONTH: Aug

YEAR: 2001

CD1d-restricted NKT cells: an interstrain comparison. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

CD1d-restricted NKT cells: an interstrain comparison. Keywords Mesh Terms:

KEYWORDS: T-Lymphocyte Subsets

MESH TERMS: metabolism

Chemical & Substance for Abstract: CD1d-restricted NKT cells: an interstrain comparison. Information

Substance Name: Killer Cell Lectin-Like Receptor Subfami

Registry Number: 145113-62-8

Grant and Affiliation Information for CD1d-restricted NKT cells: an interstrain comparison.

AFFILIATION: Department Pathology and Immunology, Monash University, Commercial Road, Prahran, Melbourne, VIC 3181, Australia.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

CD1d-restricted NKT cells: an interstrain comparison Related Publications

• Age Distribution in Milk-Borne Outbreaks of Scarlet Fever and Diphtheria.
• Asthma guidelines for children--are they useful?--no!
• CD1d-restricted NKT cells: an interstrain comparison.
• Comparative Value of State Districts and County Districts as the Basis of Local Health Organization.
• Developmental mismatch: consequences for later cardiorespiratory health.
• Dietary approaches to reduce blood pressure in a community setting: a randomised crossover study.
• Diseases causing cough.
• Education and Training of the Physician for a Public Health Career.
• Exercise studies in children using the indirect (CO 2 ) Fick method.
• Health for Three-Thirds of the Nation.
• Hemoptysis in children.
• Impact of glucose levels on advanced glycation end products in hemodialysis.
• NKT cells are phenotypically and functionally diverse.
• Optimal markers for real-time quantitative reverse transcription PCR detection of circulating tumor cells from melanoma, breast, colon, esophageal, head and neck, and lung cancers.
• PERSONAL REMINISCENCES OF A FIELD AMBULANCE DENTAL OFFICER.
• Percutaneous embolization of a left-sided varicocele in a patient with a duplicated inferior vena cava.
• Practical Uses of Diphtheria Immunization Records.
• Prediction of lymph node metastasis by analysis of gene expression profiles in primary lung adenocarcinomas.
• Preparation of semisynthetic coumermycin A1 derivatives. 3. Aromatic and heteroaromatic derivatives of 3-amino-4-hydroxy-8-methyl-7-[3-O-(5-methyl-2-pyrrolylcarbonyl) noviosyloxy]coumarin.
• Preparation of semisynthetic coumermycin A1 derivatives. IV. Aliphatic acyl and related derivatives of 3-amino-4-hydroxy-8-methyl-7-[3-O-(5-methyl-2-pyrrolylcarbonyl) noviosyloxy] coumarin.
• Preparation of semisynthetic kasugamycin derivatives. I. Aliphatic amidino derivatives of kasuganobiosamine.
• Report of the Committee on Professional Education (Proposed Report on the Educational and Experience Qualifications of Laboratory Workers).
• Robot angiography. A preliminary report.
• Rôle of the Health Department in the Prevention of Accidents.
• Spectral response of cotton aphid- (homoptera: aphididae) and spider mite- (acari: Tetranychidae) infested cotton: controlled studies.
• Study in the Epidemiology of Diphtheria in Relation to the Active Immunization of Certain Age Groups.
• THE ADMINISTRATIVE CONTROL OF MEASLES.
• THE ROUTINE DETERMINATION OF URINARY 4-HYDROXY-3-METHOXY MANDELIC ACID.
• The Age Distribution of Communicable Disease According to Size of Community.
• Variation in the timing of multiple ovulations following gonadotropin releasing hormone treatment and its relevance to collecting pronuclear embryos of sheep.