CD133 (AC133) expression on AML cells and progenitors.

CD133 (AC133) expression on AML cells and progenitors. Research Abstract Details 

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CD133 (AC133) expression on AML cells and progenitors. Abstract Text:

BACKGROUND: AML blasts differ in their functional capability, creating a hierarchy of progenitors. CD133 (AC133) is a newly described transmembrane protein expressed on CD34(+) and CD34(-) normal progenitors. We characterized the prognostic significance of CD133 expression in AML and expression of CD133 on AML progenitors thought to be responsible for maintaining this disease. METHODS: AML cells from 102 patients were analyzed for CD133 and CD34 expression, and correlated with outcome in 92 treated patients. AML cells were also FACS sorted into CD34(+)/CD133(+), CD34(+)/CD133(-), CD34(-)/CD133(+) and CD34(-)/CD133(-) subfractions, and assayed in vitro in colony-forming assay (CFU) and in suspension culture (SC) assay for up to 8 weeks, and in vivo in non-obese diabetic (NOD)/SCID mice to determine the phenotype of progenitors detected in these assays. RESULTS: CD133 expression was not correlated with event-free or overall survival, FAB subtype, cytogenetic abnormality or WBCC, but was correlated with CD34 expression. Primary AML CFU were present in all four sorted fractions. After an increasing period of time in SC, a higher proportion of cells capable of forming leukemic CFU were found in the CD34(+)/CD133(+) subfraction. Cells capable of producing leukemic engraftment in NOD/SCID mice were found in all subfractions, including the CD34(-)/CD133(-) subfraction in many patients. DISCUSSION: CD133 is not useful as a prognostic marker in AML. CD133 is expressed with CD34 on most primitive leukemic progenitors detected in vitro, however, in vivo progenitors could not be purified using CD133 in these patients.

CD133 (AC133) expression on AML cells and progenitors. Publishing Authors By Initials

For similar diagnosis: prognosis research abstracts see: diagnosis: prognosis research

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CD133 (AC133) expression on AML cells and progenitors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cytotherapy

VOLUME: 3

Page Numbers: 449-59

Journal Abbreviation: Cytotherapy

ISSN: 1465-3249

DAY: 24

MONTH: 04

YEAR: 2001

CD133 (AC133) expression on AML cells and progenitors. Information

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LANGUAGE: eng

NlmUniqueID: 100895309

CD133 (AC133) expression on AML cells and progenitors. Keywords Mesh Terms:

KEYWORDS: Prognosis

MESH TERMS: metabolism

Chemical & Substance for Abstract: CD133 (AC133) expression on AML cells and progenitors. Information

Substance Name: Membrane Proteins

Registry Number: 0

Grant and Affiliation Information for CD133 (AC133) expression on AML cells and progenitors.

AFFILIATION: Terry Fox Laboratory, British Columbia Cancer Agency Vancouver Hospital and Health Sciences Centre, Vancouver, BC Canada.

Country: England

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MEDLINETA: Cytotherapy

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