Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes.

Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Research Abstract Details 

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Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Abstract Text:

Connexin43 (Cx43) has been reported to interact with caveolin (Cav)-1, but the role of this association and whether other members of the caveolin family bind Cx43 had yet to be established. In this study, we show that Cx43 coimmunoprecipitates and colocalizes with Cav-1 and Cav-2 in rat epidermal keratinocytes. The colocalization of Cx43 with Cav-1 was confirmed in keratinocytes from human epidermis in vivo. Our mutation and Far Western analyses revealed that the C-terminal tail of Cx43 is required for its association with Cavs and that the Cx43/Cav-1 interaction is direct. Our results indicate that newly synthesized Cx43 interacts with Cavs in the Golgi apparatus and that the Cx43/Cavs complex also exists at the plasma membrane in lipid rafts. Using overexpression and small interfering RNA approaches, we demonstrated that caveolins regulate gap junctional intercellular communication (GJIC) and that the presence of Cx43 in lipid raft domains may contribute to the mechanism modulating GJIC. Our results suggest that the Cx43/Cavs association occurs during exocytic transport, and they clearly indicate that caveolin regulates GJIC.

Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Publishing Authors By Initials

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Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular biology of the cell

VOLUME: 19

Page Numbers: 912-28

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 27

MONTH: 12

YEAR: 2007

Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes.

AFFILIATION: Departments of *Anatomy and Cell Biology, Physiology and Pharmacology, and Surgery, University of Western Ontario, London, ON N6A 5C1, Canada.

Country: United States

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MEDLINETA: Mol Biol Cell

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