Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells.

Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Research Abstract Details 

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Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Abstract Text:

Takahito Moriyama,J Pablo Marquez,Tetsuro Wakatsuki,Andrey Sorokin,

In recent years, BK virus (BKV) nephritis after renal transplantation has become a severe problem. The exact mechanisms of BKV cell entry and subsequent intracellular trafficking remain unknown. Since human renal proximal tubular epithelial cells (HRPTEC) represent a main natural target of BKV nephritis, analysis of BKV infection of HRPTEC is necessary to obtain additional insights into BKV biology and to develop novel strategies for the treatment of BKV nephritis. We coincubated HRPTEC with BKV and the cholesterol-depleting agents methyl beta cyclodextrin (MBCD) and nystatin (Nys), drugs inhibiting caveolar endocytosis. The percentage of infected cells (detected by immunofluorescence) and the cellular levels of BKV large T antigen expression (detected by Western blot analysis) were significantly decreased in both MBCD- and Nys-treated HPRTEC compared to the level in HRPTEC incubated with BKV alone. HRPTEC infection by BKV was also tested after small interfering RNA (siRNA)-dependent depletion of either the caveolar structural protein caveolin-1 (Cav-1) or clathrin, the major structural protein of clathrin-coated pits. BKV infection was inhibited in HRPTEC transfected with Cav-1 siRNA but not in HRPTEC transfected with clathrin siRNA. The colocalization of labeled BKV particles with either Cav-1 or clathrin was investigated by using fluorescent microscopy and image cross-correlation spectroscopy. The rate of colocalization of BKV with Cav-1 peaked at 4 h after incubation. Colocalization with clathrin was insignificant at all time points. These results suggest that BKV entered into HRPTEC via caveolae, not clathrin-coated pits, and that BKV is maximally associated with caveolae at 4 h after infection, prior to relocation to a different intracellular compartment.

Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Publishing Authors By Initials

T Moriyama,JP Marquez,T Wakatsuki,A Sorokin,

For similar polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research abstracts see: polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research

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Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 81

Page Numbers: 8552-62

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 6

MONTH: 06

YEAR: 2007

Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Keywords Mesh Terms:

KEYWORDS: beta-Cyclodextrins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. Information

Substance Name: Nystatin

Registry Number: 1400-61-9

Grant and Affiliation Information for Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells.

AFFILIATION: Division of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL 022563

ACRONYM: HL

MEDLINETA: J Virol

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