Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing.

Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Research Abstract Details 

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Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Abstract Text:

Andrew J Love,Janet Laird,Justin Holt,Andrew J Hamilton,Ari Sadanandom,Joel J Milner,Andrew J Love,Janet Laird,Justin Holt,Andrew J Hamilton,Ari Sadanandom,Joel J Milner,Andrew J Love,Janet Laird,Justin Holt,Andrew J Hamilton,Ari Sadanandom,Joel J Milner,

We infected a transgenic Arabidopsis line (GxA), containing an amplicon-silenced 35S : : GFP transgene, with cauliflower mosaic virus (CaMV), a plant pararetrovirus with a DNA genome. Systemically infected leaves showed strong GFP fluorescence and amplicon transcripts were detectable in Northern blots, indicating that silencing of GFP had been suppressed during CaMV-infection. Transgenic Arabidopsis lines expressing CaMV protein P6, the major genetic determinant of symptom severity, were crossed with GxA. Progeny showed strong GFP fluorescence throughout and amplicon transcripts were detectable in Northern blots, indicating that P6 was suppressing local and systemic silencing. However, levels of 21 nt siRNAs derived from the GFP transgene were not reduced. In CaMV-infected plants, the P6 transgene did not reduce levels of CaMV leader-derived 21 and 24 nt siRNAs relative to levels of CaMV 35S RNA. These results demonstrate that CaMV can efficiently suppress silencing of a GFP transgene, and that P6 acts as a silencing suppressor.

Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Publishing Authors By Initials

AJ Love,J Laird,J Holt,AJ Hamilton,A Sadanandom,JJ Milner,AJ Love,J Laird,J Holt,AJ Hamilton,A Sadanandom,JJ Milner,AJ Love,J Laird,J Holt,AJ Hamilton,A Sadanandom,JJ Milner,

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Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of general virology

VOLUME: 88

Page Numbers: 3439-44

Journal Abbreviation: J. Gen. Virol.

ISSN: 0022-1317

DAY: 20

MONTH: Dec

YEAR: 2007

Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Information

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LANGUAGE: eng

NlmUniqueID: 77340

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Grant and Affiliation Information for Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing.

AFFILIATION: Plant Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.

Country: England

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MEDLINETA: J Gen Virol

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