CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity.

CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Research Abstract Details 

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CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Abstract Text:

Hang Chen,Carol Macleod,Bijia Deng,Lawrence Mason,Marion Kasaian,Samuel Goldman,Stan Wolf,Cara Williams,Michael R Bowman,Hang Chen,Carol Macleod,Bijia Deng,Lawrence Mason,Marion Kasaian,Samuel Goldman,Stan Wolf,Cara Williams,Michael R Bowman,

We investigated the effect the loss of the CAT-2 gene (CAT-2-/-) has on lung resistance (R(L)) and tracheal isometric tension. The R(L) of CAT-2-/- mice at a maximal dose of acetylcholine (ACh) was decreased by 33.66% (P = 0.05, n = 8) compared with that of C57BL/6 (B6) mice. The isometric tension of tracheal rings from CAT-2-/- mice showed a significant decrease in carbachol (CCh)-induced force generation (33.01%, P < 0.05, n = 8) compared with controls. The isoproterenol- or the sodium nitroprusside-induced relaxation was not affected in tracheal rings from CAT-2-/- mice. The activity of iNOS and arginase in lung tissue lysates of CAT-2-/- mice was indistinguishable from that of B6 mice. Furthermore, the expression of phospholipase-Cbeta (PLC-beta) and phosphatidylinositol-(4)-phosphate-5-kinase-gamma (PIP-5K-gamma) was examined in the lung tissue of CAT-2-/- and B6 mice. The expression of PIP-5K-gamma but not PLC-beta was significantly reduced in CAT-2-/- compared with B6 mice. The reduced airway smooth muscle (ASM) contractility to CCh seen in the CAT-2-/- tracheal rings was completely reversed by pretreating the rings with 100 muM spermine. This increase in the CAT-2-/- tracheal ring contraction upon spermine pretreatment correlated with a recovery of the expression of PIP-5K-gamma. Our data indicates that CAT-2 exerts control over ASM force development through a spermine-dependent pathway that directly correlates with the expression level of PIP-5K-gamma in the lung.

CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Publishing Authors By Initials

H Chen,C Macleod,B Deng,L Mason,M Kasaian,S Goldman,S Wolf,C Williams,MR Bowman,H Chen,C Macleod,B Deng,L Mason,M Kasaian,S Goldman,S Wolf,C Williams,MR Bowman,

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

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CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Lung cellular and

VOLUME: 293

Page Numbers: L883-91

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 20

MONTH: 07

YEAR: 2007

CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Information

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LANGUAGE: eng

NlmUniqueID: 100901229

CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: physiology

Chemical & Substance for Abstract: CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity. Information

Substance Name: Arginase

Registry Number: EC 3.5.3.1

Grant and Affiliation Information for CAT-2 amplifies the agonist-evoked force of airway smooth muscle by enhancing spermine-mediated phosphatidylinositol-(4)-phosphate-5-kinase-gamma activity.

AFFILIATION: Inflammation Department, Wyeth Research, 200 Cambridge Park Drive, Cambridge MA 02140, USA.

Country: United States

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MEDLINETA: Am J Physiol Lung Cell Mol Phy

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