Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana.

Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Research Abstract Details 

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Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Abstract Text:

Michael A Menze,Steven C Hand,

Evaluation of apoptotic processes downstream of the mitochondrion reveals caspase-9- and low levels of caspase-3-like activities in partly purified extracts of Artemia franciscana embryos. However, in contrast to experiments with extracts of human hepatoma cells, cytochrome c fails to activate caspase-3 or -9 in extracts from A. franciscana. Furthermore, caspase-9 activity is sensitive to exogenous calcium. The addition of 5 mM calcium leads to a 4.86 +/- 0.19 fold (SD) (n = 3) increase in activity, which is fully prevented with 150 mM KCl. As with mammalian systems, high ATP (>1.25 mM) suppresses caspase activity in A. franciscana extracts. A strong inhibition of caspase-9 activity was also found by GTP. Comparison of GTP-induced inhibition of caspase-9 at 0 and 2.5 mM MgCl(2) indicates that free (nonchelated) GTP is likely to be the inhibitory form. The strongest inhibition among all nucleotides tested was with ADP. Inhibition by ADP in the presence of Mg(2+) is 60-fold greater in diapause embryos than in postdiapause embryos. Because ADP does not change appreciably in concentration between the two physiological states, it is likely that this differential sensitivity to Mg(2+)-ADP is important in avoiding caspase activation during diapause. Finally, mixtures of nucleotides that mimic physiological concentrations in postdiapause and diapause states underscore the depressive action of these regulators on caspase-9 during diapause. Our biochemical characterization of caspase-like activity in A. franciscana extracts reveals that multiple mechanisms are in place to reduce the probability of apoptosis under conditions of energy limitation in this embryo.

Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Publishing Authors By Initials

MA Menze,SC Hand,

For similar chlorides: potassium chloride research abstracts see: chlorides: potassium chloride research

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Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Regulatory, integr

VOLUME: 292

Page Numbers: R2039-47

Journal Abbreviation:

ISSN: 0363-6119

DAY: 25

MONTH: 01

YEAR: 2007

Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901230

Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Keywords Mesh Terms:

KEYWORDS: Potassium Chloride

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana. Information

Substance Name: Caspases

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana.

AFFILIATION: Dept of Biological Sciences, Louisiana State Univ, Baton Rouge, LA 70803, USA. menze@lsu.edu

Country: United States

AGENCY: United States NIGMS

GRANT: 1 R01 GM071345-01

ACRONYM: GM

MEDLINETA: Am J Physiol Regul Integr Comp

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