Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Abstract Text:

Hisayo Yamaoka,Hirotaka Asato,Toru Ogasawara,Satoru Nishizawa,Tsuguharu Takahashi,Takashi Nakatsuka,Isao Koshima,Kozo Nakamura,Hiroshi Kawaguchi,Ung-il Chung,Tsuyoshi Takato,Kazuto Hoshi,

To seek a suitable scaffold for cartilage tissue engineering, we compared various hydrogel materials originating from animals, plants, or synthetic peptides. Human auricular chondrocytes were embedded in atelopeptide collagen, alginate, or PuraMatrix, all of which are or will soon be clinically available. The chondrocytes in the atelopeptide collagen proliferated well, while the others showed no proliferation. A high-cell density culture within each hydrogel enhanced the expression of collagen type II mRNA, when compared with that without hydrogel. By stimulation with insulin and BMP-2, collagen type II and glycosaminoglycan were significantly accumulated within all hydrogels. Chondrocytes in the atelopeptide collagen showed high expression of beta1 integrin, seemingly promoting cell-matrix signaling. The N-cadherin expression was inhibited in the alginate, implying that decrease in cell-to-cell contacts may maintain chondrocyte activity. The matrix synthesis in PuraMatrix was less than that in others, while its Young's modulus was the lowest, suggesting a weakness in gelling ability and storage of cells and matrices. Considering biological effects and clinical availability, atelopeptide collagen may be accessible for clinical use. However, because synthetic peptides can control the risk of disease transmission and immunoreactivities, some improvement in gelling ability would provide a more useful hydrogel for ideal cartilage regeneration.

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Publishing Authors By Initials

H Yamaoka,H Asato,T Ogasawara,S Nishizawa,T Takahashi,T Nakatsuka,I Koshima,K Nakamura,H Kawaguchi,UI Chung,T Takato,K Hoshi,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 78

Page Numbers: 1-11

Journal Abbreviation:

ISSN: 1549-3296

DAY: 19

MONTH: Jul

YEAR: 2006

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. Information

Substance Name: Insulin

Registry Number: 11061-68-0

Grant and Affiliation Information for Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.

AFFILIATION: Department of Fujisoft ABC Cartilage and Bone Regeneration, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-Ku, Tokyo 113-0033, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomed Mater Res A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials Related Publications

• A novel ceramide trihexoside from the eggs of the sea urchin, Hemicentrotus pulcherrimus.
• A novel difucosylated neutral glycosphingolipid from the eggs of the sea urchin, Hemicentrotus pulcherrimus: I. Purification and structural determination of the glycolipid.
• A novel difucosylated neutral glycosphingolipid from the eggs of the sea urchin, Hemicentrotus pulcherrimus: II. Structural determination by two-dimensional NMR.
• Automated Classification of Cerebral Arteries in MRA Images and Its Application to Maximum Intensity Projection.
• CT-based postimplant dosimetry of prostate brachytherapy: comparison of 1-mm and 5-mm section CT.
• Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.
• Ceramide dihexosides from the spermatozoa of the starfish, Asterias amurensis, consist of gentiobiosyl- cellobiosyl-, and lactosylceramide.
• Cholesterol sulphate affects production of steroid hormones by reducing steroidogenic acute regulatory protein level in adrenocortical cells.
• Comparison of urethral diameters for calculating the urethral dose after permanent prostate brachytherapy.
• Detection of in vivo proteasome activity in a starfish oocyte using membrane-impermeant substrate.
• Evaluation of conversion coefficients from measurable to risk quantities for external exposure over contaminated soil by use of physical human phantoms.
• Gangliosides from the eggs of the sea urchin, Anthocidaris crassispina.
• Glucosylceramide having a novel tri-unsaturated long-chain base from the spermatozoa of the starfish, Asterias amurensis.
• Immunocytochemical study of the distribution of a ganglioside in sea urchin eggs.
• Improving the Classification of Cirrhotic Liver by using Texture Features.
• In situ surface X-ray scattering of stepped surface of platinum: Pt(311).
• In vitro response of immunoregulatory cytokine expression in human monocytic cells to human parvovirus B19 capsid.
• Melibiosylceramide as the sole ceramide dihexoside from the eggs of the sea urchin, Anthocidaris crassispina.
• Methionine sulfoxide reductase A and a dietary supplement S-methyl-L-cysteine prevent Parkinson's-like symptoms.
• Microdosimetric evaluation of secondary particles in a phantom produced by carbon 290 MeV/nucleon ions at HIMAC.
• Milk consumption is a risk factor for prostate cancer in Western countries: evidence from cohort studies.
• Monte Carlo-based calculation of imaging plate response to (90)Sr in teeth: experimental validation of the required correction on sample thickness.
• Nodule detection in 3D chest CT images using 2nd order autocorrelation features.
• Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes.
• Ossifying pleomorphic adenoma of the parotid gland: A case report and review.
• Protoplast fusion method for efficient transformation of mouse spermatogenic cells.
• Radioactivity in atomic-bomb samples from exposure to environmental neutrons.
• Strong static magnetic field stimulates bone formation to a definite orientation in vitro and in vivo.
• [Effect of gravity on the induction of somatic mutations by radiation in Drosophila]
• [Mechanisms of bone calcification]