Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver.

Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver. Research Abstract Details 

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Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver. Abstract Text:

G J Sawyer,X Dong,M Whitehorne,A Grehan,M Seddon,A M Shah,X Zhang,J W Fabre,G J Sawyer,X Dong,M Whitehorne,A Grehan,M Seddon,A M Shah,X Zhang,J W Fabre,

Hydrodynamic gene delivery to the liver is a valuable experimental tool and an attractive option for nonviral gene therapy of liver disease. However, little attention has been paid to the major obstacle to clinical application: acute volume overload of the cardiovascular system. We delivered volumes of DNA solution (pGL3 plasmid) corresponding to 1, 2, 4, 6 and 8% of the body weight at 100 ml/min to the inferior vena cava (IVC) of DA strain rats. Central venous pressure (CVP), arterial pressure, pulse and electrocardiogram (ECG) were continuously recorded for subsequent analysis. Each volume produced a characteristic response, but all (including the 1% volume) caused severe falls in blood pressure and pulse within 1-2 s of the infusion, with ectopic beats and widening of the QRS complex in the ECG. The response to volumes of 4% and higher suggested that the liver acted as a volume sink, mitigating the immediate effects of volume overload. The 6 and 8% volumes caused profound and protracted falls in blood pressure and pulse, with a multitude of severe electrical abnormalities in the heart, including electromechanical dissociation. Vagal blockade with atropine, and the use of Ringer's solution to prevent electrolyte disturbances, did not ameliorate this picture.

Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver. Publishing Authors By Initials

GJ Sawyer,X Dong,M Whitehorne,A Grehan,M Seddon,AM Shah,X Zhang,JW Fabre,GJ Sawyer,X Dong,M Whitehorne,A Grehan,M Seddon,AM Shah,X Zhang,JW Fabre,

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Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene therapy

VOLUME: 14

Page Numbers: 1208-17

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 14

MONTH: 06

YEAR: 2007

Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver. Information

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LANGUAGE: eng

NlmUniqueID: 9421525

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Grant and Affiliation Information for Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver.

AFFILIATION: Department of Hepatology and Transplantation, King's College London School of Medicine, The Rayne Institute, and Department of Clinical Perfusion Sciences, King's College Hospital, London, UK.

Country: England

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MEDLINETA: Gene Ther

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