Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen.

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Abstract Text:

K Hino,S Yamamoto,O Sano,Y Taniguchi,K Kohno,M Usui,S Fukuda,H Hanzawa,H Haruyama,M Kurimoto,

The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides. [formula: see text]

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Publishing Authors By Initials

K Hino,S Yamamoto,O Sano,Y Taniguchi,K Kohno,M Usui,S Fukuda,H Hanzawa,H Haruyama,M Kurimoto,

For similar carbohydrates: monosaccharides: pentoses: xylose research abstracts see: carbohydrates: monosaccharides: pentoses: xylose research

PUBMED ID PMID:

MEDLINE DATE:

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 289-95

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1995

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Keywords Mesh Terms:

KEYWORDS: Xylose

MESH TERMS: analysis

Chemical & Substance for Abstract: Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen.

AFFILIATION: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar Cryptomeria japonica pollen Related Publications

• Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen.
• Determinants of patient satisfaction with photodynamic therapy for neovascular age-related macular degeneration or polypoidal choroidal vasculopathy.
• Effect of hydrofluoric acid on glucose metabolism of the mouse studied by whole-body autoradiography.
• HISTOCHEMISTRY OF THE RETINA IN THE ALLOXAN-DIABETIC RAT.
• Impending rupture of a blunt trauma-induced left ventricular aneurysm: report of a case.
• Individualities of domestic fowls in the hemagglutination of vaccinia virus.
• [Mitral valvuloplasty for the repair of mitral valve prolapse due to tendon rupture of the posterior leaflet in a father and son--case reports]