Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells.

Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Research Abstract Details 

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Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Abstract Text:

S Kodama,T Endo,N Tsuruoka,M Tsujimoto,A Kobata,

The asparagine-linked sugar chains of recombinant human interleukin 5 produced by Chinese hamster ovary cells were released quantitatively as oligosaccharides by hydrazinolysis. After N-acetylation followed by NaB3H4 reduction, each oligosaccharide was isolated by paper electrophoresis and serial lectin column chromatography. Study of their structures by sequential exoglycosidase digestion in combination with methylation analysis, revealed that they are bi-, tri-, and tetraantennary complex-type with fucosylated and non-fucosylated trimannosyl cores and high mannose type sugar chains. More than 80% of the sugar chains occur as biantennary complex-type sugar chains. Although acidic oligosaccharides amount to only 14% of the total oligosaccharides, their sialic acid residues occur exclusively as the Sia alpha 2----3Gal group. Removal of the sugar moiety from intact recombinant human interleukin 5 produced a 2.5-fold increase of its activity to induce IgM secretion.

Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Publishing Authors By Initials

S Kodama,T Endo,N Tsuruoka,M Tsujimoto,A Kobata,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 693-701

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1991

Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells. Information

Substance Name: Glycoside Hydrolases

Registry Number: EC 3.2.1.-

Grant and Affiliation Information for Carbohydrate structures of human interleukin 5 expressed in Chinese hamster ovary cells.

AFFILIATION: Suntory Institute for Biomedical Research, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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