Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp.

Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Research Abstract Details 

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Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Abstract Text:

I Matsui,K Oishi,

L-Fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces no. 100-2 were labeled with N-succinimidyl-[2,3-3H]propionate to investigate quantitatively their binding properties to human erythrocytes. The labeling did not influence the physical properties or the hemagglutinating activity of the lectin particles. The binding studies suggested that two kinds of receptor sites were present on the erythrocytes. Association constants (Ka's) of the lectin particles to the receptor sites and the numbers of the receptor sites (n) on human O erythrocytes were calculated to be 4.60 X 10(8) M-1 and 3.17 X 10(4)/cell for high-affinity receptor sites, and 7.5 X 10(7) M-1 and 1.33 X 10(5)/cell for low-affinity ones. The inhibition constants (Ki's) for L-fucose, p-nitrophenyl (PNP)-beta-L-fucoside, D-mannose, and PNP-alpha-D-mannoside were calculated to be 1.20 X 10(3), 1.82 X 10(3), 1.82 X 10(2), and 2.40 X 10(2) M-1, respectively. The numbers of carbohydrate-binding sites (m) on the lectin particles were estimated to be 2.82, 2.18, 2.19, and 2.21 for L-fucose, PNP-beta-L-fucoside, D-mannose, and PNP-alpha-D-mannoside, respectively, suggesting that SFL 100-2 has two carbohydrate-binding sites per particle.

Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Publishing Authors By Initials

I Matsui,K Oishi,

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Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 115-21

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1986

Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Keywords Mesh Terms:

KEYWORDS: Tritium

MESH TERMS: immunology

Chemical & Substance for Abstract: Carbohydrate-binding properties of L-fucose, D-mannose-specific lectin (SFL 100-2) particles produced by Streptomyces sp. Information

Substance Name: Fucose

Registry Number: 3713-31-3

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Country: JAPAN

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MEDLINETA: J Biochem

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