Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice.

Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Research Abstract Details 

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Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Abstract Text:

Xiaoying Lu,Lindsey C Pingel,Kindra K Burnell,Joseph E Cavanaugh,Kim A Brogden,

Mice are widely used as models to study the roles of chemokines and cytokines in immune and inflammatory responses. In our work to determine the basal levels of cytokines in saliva, nasal wash fluid (NWF), bronchoalveolar lavage fluid (BALF), and serum of mice, we found that injection of carbamoylcholine chloride, used to stimulate saliva production, induced variations in the interleukin (IL) 6 levels of NWF and BALF supernatants. To characterize this response, C57BL/6 mice were given 10 microg carbamoylcholine chloride intraperitoneally and euthanized at 0, 1, 3, 6, 12, 24, 48, 72, and 96 h after injection. IL6 was increased in NWF supernatants by 2 to 3 h, remained elevated for 24 h, and declined by 48 h after injection. To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. In 47 mice, all cytokine levels in saliva supernatants, NWF supernatants, BALF supernatants, and serum were within normal reported levels (range, 1 to 364 pg/ml); in the serum of the remaining 3 mice, GM-CSF, IL1beta, and IL2 levels were increased. In summary, carbamoylcholine chloride induces a rapid, elevated IL6 response in the nasal cavity and respiratory tract of mice but does not alter the levels of other Th1, Th2, or proinflammatory cytokines.

Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Publishing Authors By Initials

X Lu,LC Pingel,KK Burnell,JE Cavanaugh,KA Brogden,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Comparative medicine

VOLUME: 57

Page Numbers: 349-54

Journal Abbreviation: Comp. Med.

ISSN: 1532-0820

DAY: 3

MONTH: Aug

YEAR: 2007

Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100900466

Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Information

Substance Name: Carbachol

Registry Number: 51-83-2

Grant and Affiliation Information for Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice.

AFFILIATION: Department of Periodontics, College of Public Health, The University of Iowa, Iowa City, IA, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: R01 DE014390

ACRONYM: DE

MEDLINETA: Comp Med

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