Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Research Abstract Details 

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Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Abstract Text:

Kyoungtae Kim,Atsuko Yamashita,Martin A Wear,Yuichiro ,John A Cooper,

The mechanism by which capping protein (CP) binds barbed ends of actin filaments is not understood, and the physiological significance of CP binding to actin is not defined. The CP crystal structure suggests that the COOH-terminal regions of the CP alpha and beta subunits bind to the barbed end. Using purified recombinant mutant yeast CP, we tested this model. CP lacking both COOH-terminal regions did not bind actin. The alpha COOH-terminal region was more important than that of beta. The significance of CP's actin-binding activity in vivo was tested by determining how well CP actin-binding mutants rescued null mutant phenotypes. Rescue correlated well with capping activity, as did localization of CP to actin patches, indicating that capping is a physiological function for CP. Actin filaments of patches appear to be nucleated first, then capped with CP. The binding constants of yeast CP for actin suggest that actin capping in yeast is more dynamic than in vertebrates.

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Publishing Authors By Initials

K Kim,A Yamashita,MA Wear,Y ,JA Cooper,

For similar investigative techniques: genetic techniques: sequence alignment research abstracts see: investigative techniques: genetic techniques: sequence alignment research

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Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of cell biology

VOLUME: 164

Page Numbers: 567-80

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 9

MONTH: 02

YEAR: 2004

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Keywords Mesh Terms:

KEYWORDS: Sequence Alignment

MESH TERMS: metabolism

Chemical & Substance for Abstract: Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. Information

Substance Name: Saccharomyces cerevisiae Proteins

Registry Number: 0

Grant and Affiliation Information for Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.

AFFILIATION: Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM038542-15

ACRONYM: GM

MEDLINETA: J Cell Biol

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