Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain.

Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Research Abstract Details 

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Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Abstract Text:

Nalini Ramarao,Christophe Le Clainche,Tina Izard, Bourdet-Sicard,Elisabeth Ageron,Philippe J Sansonetti,Marie-France Carlier,Guy Tran Van Nhieu,

Shigella, the causative agent of bacillary dysentery, invades epithelial cells. Upon bacterial-cell contact, the type III bacterial effector IpaA binds to the cytoskeletal protein vinculin to promote actin reorganization required for efficient bacterial uptake. We show that the last 74 C-terminal residues of IpaA (A559) bind to human vinculin (HV) and promotes its association with actin filaments. Polymerisation experiments demonstrated that A559 was sufficient to induce HV-dependent partial capping of the barbed ends of actin filaments. These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin.

Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Publishing Authors By Initials

N Ramarao,C Le Clainche,T Izard,R Bourdet-Sicard,E Ageron,PJ Sansonetti,MF Carlier,G Tran Van Nhieu,

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

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Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEBS letters

VOLUME: 581

Page Numbers: 853-7

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 2

MONTH: 02

YEAR: 2007

Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Information

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LANGUAGE: eng

NlmUniqueID: 155157

Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. Information

Substance Name: Vinculin

Registry Number: 125361-02-6

Grant and Affiliation Information for Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain.

AFFILIATION: Unité de Pathogénie Microbienne Moléculaire, Inserm U786, Institut Pasteur, 75724 Paris Cedex 15, France.

Country: Netherlands

AGENCY: United States NIGMS

GRANT: GM71596

ACRONYM: GM

MEDLINETA: FEBS Lett

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