Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis.

Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Research Abstract Details 

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Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Abstract Text:

J Scott Edgar,Chaitanya P Pabbati,Robert M Lorenz,Mingyan He,Gina S Fiorini,Daniel T Chiu,

This paper demonstrates the ability to use capillary electrophoresis (CE) separation coupled with laser-induced fluorescence for analyzing the contents of single femtoliter-volume aqueous droplets. A single droplet was formed using a T-channel (3 microm wide by 3 microm tall) connected to microinjectors, and then the droplet was fluidically moved to an immiscible boundary that isolates the CE channel (50 microm wide by 50 microm tall) from the droplet generation region. Fusion of the aqueous droplet with the immiscible boundary effectively injects the droplet content into the separation channel. In addition to injecting the contents of droplets, we found aqueous samples can be introduced directly into the separation channel by reversibly penetrating and resealing the immiscible partition. Because droplet generation in channels requires hydrophobic surfaces, we have also investigated the advantages to using all hydrophobic channels versus channel systems with patterned hydrophobic and hydrophilic regions. To fabricate devices with patterned surface chemistry, we have developed a simple strategy based on differential wetting to deposit selectively a hydrophilic polymer (poly(styrenesulfonate)) onto desired regions of the microfluidic chip. Finally, we applied our device to the separation of a simple mixture of fluorescein-labeled amino acids contained within a approximately 10-fL droplet.

Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Publishing Authors By Initials

JS Edgar,CP Pabbati,RM Lorenz,M He,GS Fiorini,DT Chiu,

For similar natural sciences: physics: optics: light: luminescence: fluorescence research abstracts see: natural sciences: physics: optics: light: luminescence: fluorescence research

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Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 78

Page Numbers: 6948-54

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Oct

YEAR: 2006

Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. Keywords Mesh Terms:

KEYWORDS: Fluorescence

MESH TERMS: methods

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Grant and Affiliation Information for Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis.

AFFILIATION: Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: EB005197

ACRONYM: EB

MEDLINETA: Anal Chem

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