Capillary electrophoresis of sugar acids.

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Capillary electrophoresis of sugar acids. Abstract Text:

This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of such saccharidic compounds are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of sugar acids released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii) oligosaccharides derivatized with 4-aminobenzonitrile, arising from chemical hydrolysis of alginate; (iii) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G.

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Capillary electrophoresis of sugar acids. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 384

Page Numbers: 307-55

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 8

MONTH: 04

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 9214969

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AFFILIATION: Novartis Vaccines and Diagnostics, Siena, Italy.

Country: United States

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MEDLINETA: Methods Mol Biol

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