Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood.

Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood. Research Abstract Details 

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Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood. Abstract Text:

Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30min employing a 10mmol/l phosphate buffer, pH 2.8, 18 degrees C, 15kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r=0.995-0.999) were obtained. The method was used to analyze human capillary blood.

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Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 873

Page Numbers: 70-6

Journal Abbreviation: J. Chromatogr. B Analyt. Techn

ISSN: 1570-0232

DAY: 3

MONTH: 08

YEAR: 2008

Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood. Information

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LANGUAGE: eng

NlmUniqueID: 101139554

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Grant and Affiliation Information for Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood.

AFFILIATION: Institute for Medical Research, Military Medical Academy, 11002 Belgrade, Crnotravska 17, Serbia.

Country: Netherlands

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MEDLINETA: J Chromatogr B Analyt Technol

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