Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS.

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Abstract Text:

Ryan T Kelly,Jason S Page,Rui Zhao,Wei-Jun Qian,Heather M Mottaz,Keqi Tang,Richard D Smith,Ryan T Kelly,Jason S Page,Rui Zhao,Wei-Jun Qian,Heather M Mottaz,Keqi Tang,Richard D Smith,Ryan T Kelly,Jason S Page,Rui Zhao,Wei-Jun Qian,Heather M Mottaz,Keqi Tang,Richard D Smith,

We describe the coupling of liquid chromatography (LC) separations with mass spectrometry (MS) using nanoelectrospray ionization (nano-ESI) multiemitters. The array of 19 emitters reduced the flow rate delivered to each emitter, allowing the enhanced sensitivity that is characteristic of nano-ESI to be extended to higher flow rate separations. The signal for tryptic fragments from proteins spiked into a human plasma sample increased 11-fold on average when the multiemitters were employed, due to increased ionization efficiency and improved ion transfer efficiency through a newly designed heated multicapillary MS inlet. Additionally, the LC peak signal-to-noise ratio increased approximately 7-fold when the multiemitter configuration was used. The low dead volume of the emitter arrays preserved peak shape and resolution for robust capillary LC separations using total flow rates of 2 muL/min.

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Publishing Authors By Initials

RT Kelly,JS Page,R Zhao,WJ Qian,HM Mottaz,K Tang,RD Smith,RT Kelly,JS Page,R Zhao,WJ Qian,HM Mottaz,K Tang,RD Smith,RT Kelly,JS Page,R Zhao,WJ Qian,HM Mottaz,K Tang,RD Smith,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 80

Page Numbers: 143-9

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 29

MONTH: 11

YEAR: 2007

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS.

AFFILIATION: Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS Related Publications

• 10 do's and don'ts about tax homes.
• A gravitational lens.
• An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material.
• An electrostrictive drive for fine pitch control in double-crystal monochromators.
• Animal model of human disease: inherited erythrocyte glutathione deficiency.
• Bacterial pathogens of the respiratory tract of the newborn infant.
• Brain uptake of nonsteroidal anti-inflammatory drugs: ibuprofen, flurbiprofen, and indomethacin.
• Calculating Society's Willingness to Pay for a QALY : Key Questions for Discussion.
• Characterizing the structures and folding of free proteins using 2-D gas-phase separations: observation of multiple unfolded conformers.
• Coccidioidomycosis.
• Demoralization, anhedonia and grief in patients with severe physical illness.
• Developmental outcomes after early or delayed insertion of tympanostomy tubes.
• Effects of hormone-induced superovulation on latent infectious bovine rhinotracheitis virus in cattle.
• Euphemistic codes and tell-tale hearts: humane assistance in end-of-life cases.
• Evaluation of beta-endorphin and naloxone on bovine uterine motility.
• Gastrointestinal disease in adolescents.
• Increased amyloid- levels in APPSWE transgenic mice treated chronically with a physiological high-fat high-cholesterol diet.
• Joys of teaching nursing.
• Occupational health in India.
• Odontoblast morphology and dental repair.
• Practical management of tuberculosis.
• Preventing and treating: Heart disease in women.
• Synthesis of 4,4-difluoroglycosides using ring-closing metathesis.
• The Herpesvirus capsid surface protein, VP26, and the majority of the tegument proteins are dispensable for capsid transport toward the nucleus.
• The effects of rhBMP-2 on human osteosarcoma cells and human gingival fibroblasts in vitro.
• The effects of xylazine hydrochloride on intrauterine pressure in the cow.
• The reliability of willingness to pay for changes in health status.
• The role of primary care in infertility management.
• What is happening in the real world in lipid therapy and is it appropriate? The need for a paradigm shift.
• X-ray Imaging and Microspectroscopy of Plants and Fungi.