CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility.

CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Research Abstract Details 

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CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Abstract Text:

Mei Zhang,Jun Liu,Alan Cheng,Stephanie M Deyoung,Xiaowei Chen,Lisa H Dold,Alan R Saltiel,

CAP/Ponsin belongs to the SoHo family of adaptor molecules that includes ArgBP2 and Vinexin. These proteins possess an N-terminal sorbin homology (SoHo) domain and three C-terminal SH3 domains that bind to diverse signaling molecules involved in a variety of cellular processes. Here, we show that CAP binds to the cytoskeletal proteins paxillin and vinculin. CAP localizes to cell-extracellular matrix (ECM) adhesion sites, and this process requires binding to vinculin. Overexpression of CAP induces the aggregation of paxillin, vinculin and actin at cell-ECM adhesion sites. Moreover, CAP inhibits adhesion-dependent processes such as cell spreading and focal adhesion turnover, whereas a CAP mutant that is unable to localize to cell-ECM adhesion sites is incapable of exerting these effects. Finally, depletion of CAP by siRNA-mediated knockdown leads to enhanced cell spreading, migration and the activation of the PAK/MEK/ERK pathway in REF52 cells. Taken together, these results indicate that CAP is a cytoskeletal adaptor protein involved in modulating adhesion-mediated signaling events that lead to cell migration.

CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Publishing Authors By Initials

M Zhang,J Liu,A Cheng,SM Deyoung,X Chen,LH Dold,AR Saltiel,

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

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CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The EMBO journal

VOLUME: 25

Page Numbers: 5284-93

Journal Abbreviation: EMBO J.

ISSN: 0261-4189

DAY: 2

MONTH: 11

YEAR: 2006

CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8208664

CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility. Information

Substance Name: Extracellular Signal-Regulated MAP Kinas

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility.

AFFILIATION: Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.

Country: England

AGENCY: United States NIDDK

GRANT: F32 DK064551

ACRONYM: DK

MEDLINETA: EMBO J

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