Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide.

Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. Research Abstract Details 

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Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. Abstract Text:

Yongming Sang,M Teresa Ortega,Kristina Rune,Wang Xiau,Guolong Zhang,Jose L Soulages,Gerald H Lushington,Jianwen Fang,Todd D Williams,Frank Blecha,Tonatiuh Melgarejo,Yongming Sang,M Teresa Ortega,Kristina Rune,Wang Xiau,Guolong Zhang,Jose L Soulages,Gerald H Lushington,Jianwen Fang,Todd D Williams,Frank Blecha,Tonatiuh Melgarejo,Yongming Sang,M Teresa Ortega,Kristina Rune,Wang Xiau,Guolong Zhang,Jose L Soulages,Gerald H Lushington,Jianwen Fang,Todd D Williams,Frank Blecha,Tonatiuh Melgarejo,

Cathelicidins, a group of cationic peptides found in leukocytes and epithelial cells, play a central role in the early innate immune defense against infection. Although these host defense peptides have been reported in several mammalian species, including primates, no cathelicidins have been identified in carnivores. Here we report the cloning, tissue expression and biological activity of a novel canine cathelicidin (K9CATH). The full-length cDNA sequence of K9CATH encodes a predicted 172 amino acid pre-propeptide that is 60-70% similar to other mammalian cathelicidins. Mass spectrometry analysis confirmed that the 38 aa mature K9CATH peptide was present in neutrophil granule contents. Synthetic K9CATH displayed broad antimicrobial activity against Gram-positive bacteria (Listeria monocytogenes, and Staphylococcus aureus; MICs (minimal inhibitory concentrations) 0.5 and 50muM, respectively), Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Salmonella serotype Typhimurium, Pseudomonas aeruginosa, Proteus mirabilis; MICs 1.25muM, Salmonella serotype Enteritidis; MIC 0.5muM, and Neisseria gonorrhoeae; MIC 0.06muM), and yeast (Candida albicans; MIC 12.5-50muM). K9CATH demonstrated high antimicrobial activity against Ureaplasma canigenitalium, and lower activity against Ureaplasma urealyticum (MIC 0.06 and 50muM, respectively). Similar to its ovine congener SMAP-29, K9CATH possesses salt-independent antimicrobial activity and LPS binding capacity. K9CATH displayed minimal hemolytic activity against human, dog and chicken erythrocytes. The potency and broad antimicrobial activity of K9CATH suggest that this peptide may act as a fundamental contributor to the innate immune responses in this carnivore species.

Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. Publishing Authors By Initials

Y Sang,M Teresa Ortega,K Rune,W Xiau,G Zhang,JL Soulages,GH Lushington,J Fang,TD Williams,F Blecha,T Melgarejo,Y Sang,M Teresa Ortega,K Rune,W Xiau,G Zhang,JL Soulages,GH Lushington,J Fang,TD Williams,F Blecha,T Melgarejo,Y Sang,M Teresa Ortega,K Rune,W Xiau,G Zhang,JL Soulages,GH Lushington,J Fang,TD Williams,F Blecha,T Melgarejo,

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Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Developmental and comparative immunology

VOLUME: 31

Page Numbers: 1278-96

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ISSN: 0145-305X

DAY: 16

MONTH: 04

YEAR: 2007

Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. Information

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LANGUAGE: eng

NlmUniqueID: 7708205

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Grant and Affiliation Information for Canine cathelicidin (K9CATH): Gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide.

AFFILIATION: Departments of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.

Country: United States

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MEDLINETA: Dev Comp Immunol

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