Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis.

Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Research Abstract Details 

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Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Abstract Text:

Hosein Kouros-Mehr,Zena Werb,

The mammary gland develops in a process known as branching morphogenesis, whereby a distal epithelial bud extends and bifurcates to form an extensive ductal network. Compared with other branched organs, such as the lung and kidney, little is known about the molecular basis of branching in the mammary gland. Here we report a microarray profiling strategy to identify novel genes that may regulate mammary branching. We microdissected terminal end bud (TEB) and mature duct microenvironments from beta-actin-green fluorescent protein reporter mice and compared their RNA expression profiles with epithelium-free mammary stroma by means of microarray. We identified 1,074 genes enriched in the TEB microenvironment, 222 genes enriched in the mature duct microenvironment, and 385 genes enriched in both TEB and mature duct microenvironments. The microarray correctly predicted the expression of genes known to be enriched in the epithelium (Ets-5) and stroma (MMP-14) of TEBs and in the mature duct microenvironment (MMP-3). The microarray also correctly predicted the localization of previously uncharacterized genes, such as the TEB-enriched SPRR-1a, the duct-enriched casein-gamma, and the general epithelial marker pleiotrophin. Analysis of genes enriched in TEBs revealed several genes in the Wnt (Wnt-2, Wnt-5a, Wnt-7b, Dsh-3, Frizzled-1, Frizzled-2), hedgehog (Dhh), ephrin (Ephrin-B1, Eph-A2), and transcription factor (Twist-1, Twist-2, Snail) families. In situ hybridization verified that these genes were enriched in the TEB epithelium (Wnt-5a, Wnt-7b, Dhh, Eph-A2) or TEB stroma (Wnt-2, Frizzled-1, Ephrin-B1). We discuss the potential roles of these genes in mammary branching morphogenesis.

Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Publishing Authors By Initials

H Kouros-Mehr,Z Werb,

For similar peptides: intercellular signaling peptides and proteins: wnt proteins research abstracts see: peptides: intercellular signaling peptides and proteins: wnt proteins research

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Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Developmental dynamics : an official publication o

VOLUME: 235

Page Numbers: 3404-12

Journal Abbreviation: Dev. Dyn.

ISSN: 1058-8388

DAY: 3

MONTH: Dec

YEAR: 2006

Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201927

Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Keywords Mesh Terms:

KEYWORDS: Wnt Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Information

Substance Name: Wnt Proteins

Registry Number: 0

Grant and Affiliation Information for Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis.

AFFILIATION: Department of Anatomy, Biomedical Sciences Program, University of California, San Francisco 94143-0452, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: ES012801

ACRONYM: ES

MEDLINETA: Dev Dyn

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