Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis.

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Abstract Text:

Bradley M Mitchell,Tzu G Wu,Beth E Jackson,Kirk R Wilhelmus,

PURPOSE: To compare the virulence of wild-type Candida albicans strains in a murine model of corneal candidiasis and to investigate the role of fungal filamentation in disease progression. METHODS: Scarified corneas of immunocompetent or cyclophosphamide-treated BALB/c mice were topically inoculated with one of three human isolates of C. albicans, a homozygous mutant of the pH-dependent filamentation gene rim13 or a mutant reference strain control. Mock-inoculated eyes served as negative controls. Corneal disease was categorized daily for 8 days with quantitative fungal culturing of eyes at 6 hours, 1 day, 4 days, and 8 days after infection and histopathologic examination at 1 day and 4 days after infection. RESULTS: Corneal disease severity differed significantly among wild-type strains (P < or = 0.02). The rim13(-/-) mutant Tn7-rim13 was fully attenuated, whereas the mutant control DAY286 was fully virulent. Pretreatment of mice with cyclophosphamide increased susceptibility to wild-type C. albicans and partially rescued the attenuated phenotype of the genetically deficient rim13(-/-) fungal mutant. All strains replicated with similar kinetics in vitro, and wild-type strains had similar clearance from infected eyes. Histopathologic findings correlated with disease severity. CONCLUSIONS: Wild-type strains of C. albicans that differ significantly in ocular pathogenicity correlate with the ability of yeast to produce pseudohyphae and hyphae and to invade corneal tissue. Full attenuation of the fungal rim13(-/-) mutant is the first direct demonstration of a hyphal morphogenesis-related gene as a specific virulence factor for C. albicans during corneal infection.

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Publishing Authors By Initials

BM Mitchell,TG Wu,BE Jackson,KR Wilhelmus,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

PUBMED ID PMID:

MEDLINE DATE:

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Investigative ophthalmology & visual science

VOLUME: 48

Page Numbers: 774-80

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 3

MONTH: Feb

YEAR: 2007

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: pathology

Chemical & Substance for Abstract: Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis. Information

Substance Name: Cyclophosphamide

Registry Number: 50-18-0

Grant and Affiliation Information for Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis.

AFFILIATION: Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NEI

GRANT: EY09696

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis Related Publications

• Candida albicans strain-dependent virulence and Rim13p-mediated filamentation in experimental keratomycosis.
• Capnocytophaga keratitis.
• Clinical characteristics and outcome of Candida keratitis.
• Corneal virulence of Candida albicans strains deficient in Tup1-regulated genes.
• Ecologic effects on eye banking.
• Expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis.
• Gene profiling and signaling pathways of Candida albicans keratitis.
• Prevalence and spectrum of bacterial co-infection during fungal keratitis.
• Quality assessment and microbiologic screening of donor corneas.
• Rapidly progressive cataract and iris atrophy during treatment of Acanthamoeba keratitis.
• The clinical diagnosis of microbial keratitis.
• Therapeutic interventions for herpes simplex virus epithelial keratitis.