Candida albicans biofilms produce antifungal-tolerant persister cells.

Candida albicans biofilms produce antifungal-tolerant persister cells. Research Abstract Details 

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Candida albicans biofilms produce antifungal-tolerant persister cells. Abstract Text:

Michael D LaFleur,Carol A Kumamoto,Kim Lewis,

Fungal pathogens form biofilms that are highly recalcitrant to antimicrobial therapy. The expression of multidrug resistance pumps in young biofilms has been linked to increased resistance to azoles, but this mechanism does not seem to underlie the resistance of mature biofilms that is a model of in vivo infection. The mechanism of drug resistance of mature biofilms remains largely unknown. We report that biofilms formed by the major human pathogen Candida albicans exhibited a strikingly biphasic killing pattern in response to two microbicidal agents, amphotericin B, a polyene antifungal, and chlorhexidine, an antiseptic, indicating that a subpopulation of highly tolerant cells, termed persisters, existed. The extent of killing with a combination of amphotericin B and chlorhexidine was similar to that observed with individually added antimicrobials. Thus, surviving persisters form a multidrug-tolerant subpopulation. Interestingly, surviving C. albicans persisters were detected only in biofilms and not in exponentially growing or stationary-phase planktonic populations. Reinoculation of cells that survived killing of the biofilm by amphotericin B produced a new biofilm with a new subpopulation of persisters. This suggests that C. albicans persisters are not mutants but phenotypic variants of the wild type. Using a stain for dead cells, rare dark cells were visible in a biofilm after amphotericin B treatment, and a bright and a dim population were physically sorted from this biofilm. Only the dim cells produced colonies, showing that this method allows the isolation of yeast persisters. Given that persisters formed only in biofilms, mutants defective in biofilm formation were examined for tolerance of amphotericin B. All of the known mutants affected in biofilm formation were able to produce normal levels of persisters. This finding indicates that attachment rather than formation of a complex biofilm architecture initiates persister formation. Bacteria produce multidrug-tolerant persister cells in both planktonic and biofilm populations, and it appears that yeasts and bacteria have evolved analogous strategies that assign the function of survival to a small part of the population. In bacteria, persisters are dormant cells. It remains to be seen whether attachment initiates dormancy that leads to the formation of fungal persisters. This study suggests that persisters may be largely responsible for the multidrug tolerance of fungal biofilms.

Candida albicans biofilms produce antifungal-tolerant persister cells. Publishing Authors By Initials

MD LaFleur,CA Kumamoto,K Lewis,

For similar genetic phenomena: phenotype research abstracts see: genetic phenomena: phenotype research

PUBMED ID PMID:

MEDLINE DATE:

Candida albicans biofilms produce antifungal-tolerant persister cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Antimicrobial agents and chemotherapy

VOLUME: 50

Page Numbers: 3839-46

Journal Abbreviation: Antimicrob. Agents Chemother.

ISSN: 0066-4804

DAY: 21

MONTH: 08

YEAR: 2006

Candida albicans biofilms produce antifungal-tolerant persister cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 315061

Candida albicans biofilms produce antifungal-tolerant persister cells. Keywords Mesh Terms:

KEYWORDS: Phenotype

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Candida albicans biofilms produce antifungal-tolerant persister cells. Information

Substance Name: 3',6'-diacetylfluorescein

Registry Number: 596-09-8

Grant and Affiliation Information for Candida albicans biofilms produce antifungal-tolerant persister cells.

AFFILIATION: Department of Biology, Northeastern University, 360 Huntington Ave., 134 Mugar Hall, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI052805

ACRONYM: AI

MEDLINETA: Antimicrob Agents Chemother

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